Modifying secretion and post-translational processing in insect cells

被引：104
|
作者：
Ailor, E [1 ]
Betenbaugh, MJ [1 ]
机构：
[1] Johns Hopkins Univ, Dept Chem Engn, Baltimore, MD 21218 USA
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 1999年 / 10卷 / 02期
关键词：
D O I：
10.1016/S0958-1669(99)80024-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The baculovirus-insect cell system is a valuable tool for the expression of heterologous proteins. Due to limitations in the intracellular processing environment, however, heterologous secreted and membrane proteins are often insoluble, poorly processed, or contain 'non-human' modifications. Recent attempts to modify the insect cell secretory pathway by overexpressing processing factors have demonstrated the potential to overcome these limitations.
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页码：142 / 145
页数：4
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