Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence

cose (HG)-induced glomerular mesangial cells (GMCs) senescence. Methods: HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. beta-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot. Results: Treatment with high concentrations of glucose induced GMC senescence accompanied by shortened telomere length and increase of beta-galactosidase staining as well as P53 protein, which was abrogated after application of caveolin-1-siRNA. Conclusions: This study proved that HG induced cell senescence in GMCs. The caveolin-1 is involved in HG-induced mesangial cell senescence, and blocking caveolin- 1 significantly reduced cell senescence. The effect of caveolin-1 is mediated by P-53 pathway.