Biomarker-based assessment of the muscle maintenance and energy status of anurans from an extremely seasonal semi-arid environment, the Brazilian Caatinga

Strongly seasonal environments pose challenges for performance and survival of animals, especially when resource abundance seasonally fluctuates. We investigated the seasonal variation of key metabolic biomarkers in the muscles of males from three species (Rhinella jimi, R. granulosa and Pleurodema diplolister) of anurans from the drastically seasonal Brazilian semi-arid area, Caatinga. We examined the expression of proteins regulating energy turnover (AMP-activated protein kinase [AMPK] and protein kinase B [AKT]), protein synthesis and homeostasis (total and phosphorylated eukaryotic initiation factor 2 alpha [eIF2 alpha and p-eIF2 alpha] and chaperone proteins [HSP 60, 70, and 901) in muscles predominantly related to reproduction and locomotion. Cytochrome c oxidase (COX) activity was also assessed as an index of the muscle aerobic capacity. The expression pattern of metabolic biomarkers indicates that the maintenance of muscular function is regulated in a species-specific manner during the drastic seasonal variation. Rhinella jimi and R. granulosa that remain active during the drought appear to maintain muscles through more energy expensive pathways including elevated protein synthesis, while the aestivating P. diplolister employs energy conservation strategy suppressing protein synthesis, decreasing chaperone expression and increasing expression of AMPK. Two (P. diplolister and R. granulosa) of the three studied species activate cell survival pathways during the drought likely to prevent muscle atrophy, and all three studied species maintain the muscle aerobic capacity throughout the year, despite the resource limitation. These strategies are important considering the unpredictability of the reproductive event and high demand on muscular activity during the reproductive season in these amphibians. Summary statement We studied seasonal variation of key metabolic biomarkers in the muscles of anurans that experience drastic variation in environmental conditions and differ in seasonal activity patterns.