Endoplasmic reticulum aminopeptidase 2 gene single nucleotide polymorphisms in association with susceptibility to ankylosing spondylitis in an Iranian population

被引:6
|
作者
Ebrazeh, Mehrdad [1 ]
Nojavan, Mohammad [2 ]
Abdi-Shayan, Shiva [3 ]
Salimifard, Sevda [4 ]
Dolatshahi, Elahe [1 ]
Aslani, Saeed [5 ]
Hemmatzadeh, Maryam [6 ]
Babaie, Farhad [7 ]
Ghanavatinejad, Alireza [8 ]
Azizi, Gholamreza [1 ]
Jadidi-Niaragh, Farhad [3 ,9 ]
Zamani, Neda [10 ]
Mohammadi, Hamed [1 ,11 ]
机构
[1] Alborz Univ Med Sci, Noncommunicable Dis Res Ctr, Karaj, Iran
[2] Alfa Med Lab, Dept Lab Med, Orumiyeh, Iran
[3] Tabriz Univ Med Sci, Sch Med, Dept Immunol, Tabriz, Iran
[4] Mashhad Univ Med Sci, Fac Med, Dept Hematol & Blood Transfus, Mashhad, Razavi Khorasan, Iran
[5] Univ Tehran Med Sci, Sch Med, Dept Immunol, Tehran, Iran
[6] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran
[7] Urmia Univ Med Sci, Cellular & Mol Res Ctr, Orumiyeh, Iran
[8] Pasteur Inst Iran, Dept Immunol, Tehran, Iran
[9] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran
[10] Islamic Azad Univ, Dept Cell & Mol Biol, Marand Branch, Marand, Iran
[11] Alborz Univ Med Sci, Sch Med, Dept Immunol, Karaj, Iran
关键词
Human leukocyte antigen-B27; Ankylosing spondylitis; Inflammation; Single nucleotide polymorphisms; Endoplasmic reticulum aminopeptidase; ERAP1; PATHOGENESIS; INSIGHTS; MECHANISM; HLA-B27; ROLES; CELLS;
D O I
10.1016/j.imlet.2020.04.015
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background: Ankylosing spondylitis (AS) is a chronic autoimmune disease, in which genetic polymorphisms are critically important in establishing inflammatory state. Endoplasmic reticulum aminopeptidase (ERAP) 2 gene has been implied to be involved in AS etiopathogenesis. The current study evaluated the association of ERAP2 gene single nucleotide polymorphisms (SNPs) with susceptibility to AS in an Iranian population. Methods: Two hundred and forty AS patients and 240 healthy individuals were recruited. DNA extraction was performed from whole blood samples and RNA content was isolated from peripheral blood mononuclear cells (PBMCs). Real-time allelic discrimination approach was exerted to genotype all subjects for rs2910686, rs2248374, and rs2549782 SNPs. After cDNA synthesis, mRNA expression of cytokines was determined. Enzymelinked immunosorbent assay (ELISA) was exerted to evaluate the cytokine levels in serum of participants. Results: None of the SNPs were associated with AS risk in the whole population. However, allele and heterozygote genotype of rs2910686 SNP were associated significantly with higher risk of AS in Human leukocyte antigen (HLA)-B27 positive group. mRNA expression and serum concentrations of interleukin (IL)-17A, IL-23, interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha was increased in AS patients compared with controls. Nonetheless, mRNA expression and serum levels of cytokines was not significantly different among HLA-B27 positive AS patients with different three genotypes for rs2910686 SNP. Conclusions: AlthoughERAP2 gene rs2910686 polymorphism was significantly associated with increased risk of AS susceptibility, it might not be involved in regulation of the inflammatory cytokines during AS pathogenesis.
引用
收藏
页码:97 / 105
页数:9
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