Human malignant fibrous histiocytomas in vitro:: Growth characteristics and their association with expression of mRNA for platelet-derived growth factor, transforming growth factor-alpha and their receptors
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作者:
Abdiu, A
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Linkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, SwedenLinkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, Sweden
Abdiu, A
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Walz, TM
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机构:Linkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, Sweden
Walz, TM
Nishikawa, BK
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机构:Linkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, Sweden
Nishikawa, BK
Wingren, S
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机构:Linkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, Sweden
Wingren, S
Larsson, SE
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机构:Linkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, Sweden
Larsson, SE
Wasteson, Å
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机构:Linkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, Sweden
Wasteson, Å
机构:
[1] Linkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, Sweden
[2] Linkoping Univ, Fac Hlth Sci, Dept Neurosci & Locomot, S-58185 Linkoping, Sweden
Eight human malignant fibrous histiocytomas were examined in vitro, in order to relate their growth properties to mRNA expression for platelet-derived growth factor (PDGF), PDGF receptor (PDGF-R) transforming growth factor-alpha (TGF-alpha) and the epidermal growth factor receptor (EGF-R). Reverse transcriptase-polymerase chain reaction (RT-PCR) showed that all cell lines expressed mRNA for PDGF-R alpha and/or PDGF-R beta; six cell lines expressed mRNA for the PDGF-A chain, with one cell line coexpressing PDGF-B chain mRNA; seven cell lines expressed mRNA for TGF-alpha whereas six cell limes expressed EGF-R mRNA. Conditioned medium from three cell lines contained PDGF; none of the cell lines released TGF-alpha. Two cell lines grew without serum requirements; whereas both expressed mRNA for PDGF, PDGF-R, TGF-alpha and EGF-R other cell lines, unable to grow without serum, showed the same combination of growth factor/growth factor receptor expression. The two cell lines able to grow without serum were also shown to be stimulated by the addition of PDGF-BB. These findings show that simultaneous expression of mRNA for a growth factor and its receptor does not necessarily imply an autocrine or paracrine loop. However, two of our cell Pines fulfil the requirements of possible PDGF-related autocrine and paracrine regulation. (C) 1998 Elsevier Science Ltd. All rights reserves.
机构:
MED UNIV S CAROLINA, DEPT MED, DIV RHEUMATOL & IMMUNOL, CHARLESTON, SC 29425 USAMED UNIV S CAROLINA, DEPT MED, DIV RHEUMATOL & IMMUNOL, CHARLESTON, SC 29425 USA
ISHIKAWA, O
LEROY, EC
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MED UNIV S CAROLINA, DEPT MED, DIV RHEUMATOL & IMMUNOL, CHARLESTON, SC 29425 USAMED UNIV S CAROLINA, DEPT MED, DIV RHEUMATOL & IMMUNOL, CHARLESTON, SC 29425 USA
LEROY, EC
TROJANOWSKA, M
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MED UNIV S CAROLINA, DEPT MED, DIV RHEUMATOL & IMMUNOL, CHARLESTON, SC 29425 USAMED UNIV S CAROLINA, DEPT MED, DIV RHEUMATOL & IMMUNOL, CHARLESTON, SC 29425 USA
机构:First Mil Med Univ, Nanfang Hosp, Dept Orthopaed Surg, Guangzhou 510515, Peoples R China
Yang, DH
Jin, DD
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First Mil Med Univ, Nanfang Hosp, Dept Orthopaed Surg, Guangzhou 510515, Peoples R ChinaFirst Mil Med Univ, Nanfang Hosp, Dept Orthopaed Surg, Guangzhou 510515, Peoples R China
Jin, DD
Chen, JT
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机构:First Mil Med Univ, Nanfang Hosp, Dept Orthopaed Surg, Guangzhou 510515, Peoples R China
Chen, JT
Jing, ZS
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机构:First Mil Med Univ, Nanfang Hosp, Dept Orthopaed Surg, Guangzhou 510515, Peoples R China
Jing, ZS
Wu, DL
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机构:First Mil Med Univ, Nanfang Hosp, Dept Orthopaed Surg, Guangzhou 510515, Peoples R China