Human malignant fibrous histiocytomas in vitro:: Growth characteristics and their association with expression of mRNA for platelet-derived growth factor, transforming growth factor-alpha and their receptors

被引:15
|
作者
Abdiu, A [1 ]
Walz, TM
Nishikawa, BK
Wingren, S
Larsson, SE
Wasteson, Å
机构
[1] Linkoping Univ, Fac Hlth Sci, Dept Biomed & Surg, S-58185 Linkoping, Sweden
[2] Linkoping Univ, Fac Hlth Sci, Dept Neurosci & Locomot, S-58185 Linkoping, Sweden
关键词
fibrous histiocytoma; cultured tumour cells; platelet-derived growth factor; transforming growth factor-alpha; messenger RNA; polymerase chain reaction; serum-free culture media; conditioned culture media;
D O I
10.1016/S0959-8049(98)00247-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Eight human malignant fibrous histiocytomas were examined in vitro, in order to relate their growth properties to mRNA expression for platelet-derived growth factor (PDGF), PDGF receptor (PDGF-R) transforming growth factor-alpha (TGF-alpha) and the epidermal growth factor receptor (EGF-R). Reverse transcriptase-polymerase chain reaction (RT-PCR) showed that all cell lines expressed mRNA for PDGF-R alpha and/or PDGF-R beta; six cell lines expressed mRNA for the PDGF-A chain, with one cell line coexpressing PDGF-B chain mRNA; seven cell lines expressed mRNA for TGF-alpha whereas six cell limes expressed EGF-R mRNA. Conditioned medium from three cell lines contained PDGF; none of the cell lines released TGF-alpha. Two cell lines grew without serum requirements; whereas both expressed mRNA for PDGF, PDGF-R, TGF-alpha and EGF-R other cell lines, unable to grow without serum, showed the same combination of growth factor/growth factor receptor expression. The two cell lines able to grow without serum were also shown to be stimulated by the addition of PDGF-BB. These findings show that simultaneous expression of mRNA for a growth factor and its receptor does not necessarily imply an autocrine or paracrine loop. However, two of our cell Pines fulfil the requirements of possible PDGF-related autocrine and paracrine regulation. (C) 1998 Elsevier Science Ltd. All rights reserves.
引用
收藏
页码:2094 / 2100
页数:7
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