Lactobacillus plantarum Surface-Displayed ASFV (p14.5) Can Stimulate Immune Responses in Mice

被引：10

作者：

Huang, Quntao ^{[1
,2
,3
]}

Niu, Tianming ^{[1
,2
,3
]}

Zou, Boshi ^{[1
,2
,3
]}

Wang, Junhong ^{[1
,2
,3
]}

Xin, Junhong ^{[1
,2
,3
]}

Niu, Hui ^{[1
,2
,3
]}

Li, Nan ^{[1
,2
,3
]}

Jiang, Yuxin ^{[1
,2
,3
]}

Bao, Junfu ^{[1
,2
,3
]}

Zhang, Di ^{[1
,2
,3
]}

Feng, Xize ^{[1
,2
,3
]}

Sun, Tingting ^{[1
]}

Wang, Xin ^{[1
]}

Yang, Kaidian ^{[1
,2
,3
]}

Wang, Ying ^{[1
,2
,3
]}

Yang, Guilian ^{[1
,2
,3
]}

Zhao, Dandan ^{[2
]}

Wang, Chunfeng ^{[1
,2
,3
]}

机构：

[1] Jilin Agr Univ, Coll Vet Med, Changchun 130118, Peoples R China

[2] Jilin Agr Univ, Jilin Prov Engn Res Ctr Anim Probiot, Jilin Prov Key Lab Anim Microecol & Hlth Breeding, Changchun 130118, Peoples R China

[3] Jilin Agr Univ, Key Lab Anim Prod & Prod Qual Safety, Minist Educ, Changchun 130118, Peoples R China

来源：

VACCINES | 2022年 / 10卷 / 03期

基金：

中国国家自然科学基金;

关键词：

African Swine Fever Virus (ASFV); p14.5; CTA1-DD; IL-33; L; plantarum; immune evaluation;

D O I：

10.3390/vaccines10030355

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

African Swine Fever Virus (ASFV) has spread worldwide, and the lack of vaccines severely negatively impacts the pig industry. In this study, the p14.5 protein encoded by ASFV was used as the antigen, and the p14.5 gene was expressed in vitro using the Lactobacillus expression system. Three new functionally recombinant Lactobacillus plantarum (L. plantarum) were constructed and the expressions of the p14.5 protein, p14.5-IL-33-Mus fusion protein and CTA1-p14.5-D-D fusion protein were successfully detected using Western blot analysis. After oral immunization of SPF mice with recombinant L. plantarum, flow cytometry and ELISA were performed to detect the differentiation and maturity of T lymphocytes, B lymphocytes and DCs of the mice, which were higher than those of the control group. Specific antibodies were produced. The immunogenicity of the adjuvant group was stronger than that of the single antigen group, and the IL-33 adjuvant effect was stronger than that of the CTA1-DD adjuvant.

引用

页数：10

共 34 条

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