Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species

被引：2

作者：

Ansari, Talat Hojat ^{[1
]}

Bertozzi, Terry ^{[2
,3
,4
]}

Hacking, Jessica ^{[1
]}

Cooper, Steven J. B. ^{[2
,3
,4
]}

Gardner, Michael G. ^{[1
,2
]}

机构：

[1] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia

[2] S Australian Museum, Evolutionary Biol Unit, Adelaide, SA 5000, Australia

[3] Univ Adelaide, Australian Ctr Evolutionary Biol & Biodivers, Adelaide, SA 5005, Australia

[4] Univ Adelaide, Sch Biol Sci, Adelaide, SA 5005, Australia

来源：

AUSTRALIAN JOURNAL OF ZOOLOGY | 2014年 / 62卷 / 06期

关键词：

DNA markers; phylogeography; squamate; NON-MODEL ORGANISMS; MARKERS; PHYLOGEOGRAPHY;

D O I：

10.1071/ZO14085

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a 'northern' lineage (48 loci) and a 'southern' lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species.

引用

页码：515 / 518

页数：4