Impact of Bioreactor Geometry on Mesenchymal Stem Cell Production in Stirred-Tank Bioreactors

被引：9
|
作者：
Petry, Florian [1 ]
Salzig, Denise [1 ]
机构：
[1] Univ Appl Sci Mittelhessen, Inst Bioproc Engn & Pharmaceut Technol, Wiesenstr 14, D-35390 Giessen, Germany
来源：
CHEMIE INGENIEUR TECHNIK | 2021年 / 93卷 / 10期
关键词：
Advanced therapy medicinal products; hMSC spheroids; Hydrodynamics; Microcarriers; Shear stress; MEASLES-VIRUS INFECTION; SINGLE-USE BIOREACTORS; STROMAL CELLS; HYDRODYNAMIC STRESS; SPHEROID CULTURE; SHEAR-STRESS; SCALE-UP; EXPANSION; MICROCARRIERS; AGITATION;
D O I：
10.1002/cite.202100041
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
Human mesenchymal stem cells (hMSCs) are manufactured as cell therapy products. This requires a tightly controlled and fully understood production process, usually taking place in a stirred-tank bioreactor. In this review, the impact of bioreactor geometry as well as equipment-related parameters is discussed. We provide a theoretical background for the engineering of hMSC spheroids with a defined size and evaluate the process parameters needed for the microcarrier-based expansion of hMSCs. Finally, and based on experimental data, ideal bioreactor setups for each type of process are recommended.
引用
收藏
页码：1537 / 1554
页数：18
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