High-throughput method for the quantitation of metabolites and co-factors from homocysteine-methionine cycle for nutritional status assessment

被引：17

作者：

Da Silva, Laeticia ^{[1
]}

Collino, Sebastiano ^{[1
]}

Cominetti, Ornella ^{[1
]}

Martin, Francois-Pierre ^{[1
]}

Montoliu, Ivan ^{[1
]}

Moreno, Sergio Oller ^{[1
]}

Corthesy, John ^{[1
]}

Kaput, Jim ^{[1
]}

Kussmann, Martin ^{[1
]}

Monteiro, Jacqueline Pontes ^{[2
]}

Guiraud, Seu Ping ^{[1
]}

机构：

[1] Nestle Inst Hlth Sci SA, Campus EPFL, CH-1015 Lausanne, Switzerland

[2] Univ Sao Paulo, Dept Pediat, Fac Med Nutr & Metab, Bandeirantes Ave,HCFMRP Campus USP 3900, BR-14049900 Ribeirao Preto, SP, Brazil

来源：

BIOANALYSIS | 2016年 / 8卷 / 18期

关键词：

high throughput; methionine pathway; one-carbon metabolism; red blood cells; UPLC-MS/MS; ONE-CARBON METABOLISM; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; AMINO-ACIDS; S-ADENOSYLMETHIONINE; HUMAN PLASMA; B-VITAMINS; DISEASE RISK; FOLATE; BLOOD;

D O I：

10.4155/bio-2016-0112

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: There is increasing interest in the profiling and quantitation of methionine pathway metabolites for health management research. Currently, several analytical approaches are required to cover metabolites and co-factors. Results: We report the development and the validation of a method for the simultaneous detection and quantitation of 13 metabolites in red blood cells. The method, validated in a cohort of healthy human volunteers, shows a high level of accuracy and reproducibility. Conclusion: This high-throughput protocol provides a robust coverage of central metabolites and co-factors in one single analysis and in a high-throughput fashion. In large-scale clinical settings, the use of such an approach will significantly advance the field of nutritional research in health and disease.

引用

页码：1937 / 1949

页数：13

共 13 条

[1] High-throughput method for the quantitation of metabolites and co-factors from homocysteine-methionine cycle for nutritional status assessment (vol 8, pg 1937, 2016)
Da Silva, Laeticia
Collino, Sebastiano
Cominetti, Ornella
Martin, Francois-Pierre
Montoliu, Ivan
Moreno, Sergio Oller
Corthesy, John
Kaput, Jim
Kussmann, Martin
Monteiro, Jacqueline Pontes
Guiraud, Seu Ping
[J]. BIOANALYSIS, 2016, 8 (19) : 2092 - 2092
[2] High-throughput and simultaneous quantitative analysis of homocysteine-methionine cycle metabolites and co-factors in blood plasma and cerebrospinal fluid by isotope dilution LC-MS/MS
Guiraud, Seu Ping
Montoliu, Ivan
Da Silva, Laeticia
Dayon, Loic
Galindo, Antonio Nunez
Corthesy, John
Kussmann, Martin
Martin, Francois-Pierre
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2017, 409 (01) : 295 - 305
[3] High-throughput and simultaneous quantitative analysis of homocysteine–methionine cycle metabolites and co-factors in blood plasma and cerebrospinal fluid by isotope dilution LC–MS/MS
Seu Ping Guiraud
Ivan Montoliu
Laeticia Da Silva
Loïc Dayon
Antonio Núñez Galindo
John Corthésy
Martin Kussmann
Francois-Pierre Martin
[J]. Analytical and Bioanalytical Chemistry, 2017, 409 : 295 - 305
[4] Development of a high-throughput UHPLC-MS/MS (SRM) method for the quantitation of endogenous glucagon from human plasma
Howard, James W.
Kay, Richard G.
Tan, Tricia
Minnion, James
Ghatei, Mohammad
Bloom, Steve
Creaser, Colin S.
[J]. BIOANALYSIS, 2014, 6 (24) : 3295 - 3309
[5] Validation and Automation of a High-Throughput Multitargeted Method for Semiquantification of Endogenous Metabolites from Different Biological Matrices Using Tandem Mass Spectrometry
Nandania, Jatin
Peddinti, Gopal
Pessia, Alberto
Kokkonen, Meri
Velagapudi, Vidya
[J]. METABOLITES, 2018, 8 (03):
[6] A fast, sensitive, and high-throughput method for the simultaneous quantitation of three ellagitannins from Euphorbiae pekinensis Radix in rat plasma by ultra-HPLC-MS/MS
Hou, Pengyi
Zeng, Yan
Ma, Bingjie
Wang, Xiaofan
Liu, Zhenzhen
Li, Lu
Qu, Kankan
Bi, Kaishun
Chen, Xiaohui
[J]. JOURNAL OF SEPARATION SCIENCE, 2013, 36 (15) : 2544 - 2551
[7] A novel high-throughput method for supported liquid extraction of retinol and alpha-tocopherol from human serum and simultaneous quantitation by liquid chromatography tandem mass spectrometry
Hinchliffe, Edward
Rudge, James
Reed, Paul
[J]. ANNALS OF CLINICAL BIOCHEMISTRY, 2016, 53 (04) : 434 - 445
[8] Development of a sensitive, high-throughput UPLCMS/MS method for detecting both native flavonoid compounds from Vitaflavan grape seed extract and their gut microbial metabolites
Goodrich, Katheryn
Neilson, Andrew
[J]. FASEB JOURNAL, 2013, 27
[9] Novel gradient casting method provides high-throughput assessment of blended polyester poly(lactic-co-glycolic acid) thin films for parameter optimization
Steele, Terry W. J.
Huang, Charlotte L.
Kumar, Saranya
Irvine, Scott
Boey, Freddy Yin Chiang
Loo, Joachim S. C.
Venkatraman, Subbu S.
[J]. ACTA BIOMATERIALIA, 2012, 8 (06) : 2263 - 2270
[10] A robust high-throughput sample preparation and liquid chromatography/tandem mass spectrometry method for the quantitation of β-lyase metabolites of sulfur mustard as 1,1′-sulfonylbis-[2-(methylthio)ethane] in human urine
Reddy, Muntha K.
Nixon, Christopher
Wyatt, Shane A.
Croley, Timothy R.
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2013, 27 (10) : 1128 - 1134

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