Safety assessment of potential food ingredients in canine hepatocytes

被引：7
|
作者：
Zhang, Leshuai W. [1 ]
Koci, Juraj [1 ]
Jeffery, Brett [2 ]
Riviere, Jim E. [1 ]
Monteiro-Riviere, Nancy A. [1 ]
机构：
[1] Kansas State Univ, Coll Vet Med, Dept Anat & Physiol, Manhattan, KS 66506 USA
[2] Mars Global Food Safety Ctr, Beijing 101407, Peoples R China
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2015年 / 78卷
关键词：
FULMINANT HEPATIC-FAILURE; HEAT-SHOCK PROTEINS; CITRIC-ACID; ESSENTIAL OIL; ANTIOXIDANT ACTIVITY; CYMBOPOGON-CITRATUS; RESPIRATORY-TRACT; N-ACETYLCYSTEINE; KUPFFER CELLS; CINNAMON BARK;
D O I：
10.1016/j.fct.2015.02.003
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
This research aimed to develop in vitro methods to assess hazard of canine food ingredients. Canine hepatocytes were harvested and cell viability of clove-leaf oil (CLO), eugenol (EUG), lemongrass oil (LGO), guanosine monophosphate (GMP), inosine monophosphate (IMP), sorbose, ginger-root extract (GRE), cinnamon-bark oil (CBO), cinnamaldehyde (CINA), thymol oil (TO), thymol (THYM), and citric acid were assessed with positive controls: acetaminophen (APAP), aflatoxin B1 and xylitol. Molecular Toxicology PathwayFinder array (MTPF) analyzed toxicity mechanisms for LGO. LC50 for APAP was similar among human (3.45), rat (2.35), dog (4.26 mg/ml). Aflatoxin B1 had an LC50 of 4.43 (human), 5.78 (rat) and 6.05 (dog) mu g/ml; xylitol did not decrease viability. LC50 of CLO (0.185 +/- 0.075(SD)), EUG (0.165 +/- 0.112), LGO (0.220 +/- 0.012), GRE (1.54 +/- 0.31) mg/m1; GMP (166.03 +/- 41.83), GMP + IMP (208.67 +/- 15.27) mM; CBO (0.08 +/- 0.03), CINA (0.11 +/- 0.01), TO (0.21 +/- 0.03), THYM (0.05 +/- 0.01), citric acid (1.58 +/- 0.08) mg/ml, while sorbose was non-toxic. LGO induced upregulation of 16 and down-regulation of 24 genes, which CYP and heat shock most affected. These results suggest that in vitro assays such as this may be useful for hazard assessment of food ingredients for altered hepatic function. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：105 / 115
页数：11
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