A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer

被引：2

作者：

Kanemaru, Hisashi ^{[1
]}

Mizukami, Yukari ^{[1
]}

Kaneko, Akira ^{[1
]}

Kajihara, Ikko ^{[1
]}

Fukushima, Satoshi ^{[1
]}

机构：

[1] Kumamoto Univ, Fac Life Sci, Dept Dermatol & Plast Surg, 1-1-1 Honjo, Kumamoto 8608556, Japan

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 01期

基金：

日本学术振兴会;

关键词：

IMMUNOTHERAPY;

D O I：

10.1016/j.xpro.2022.101128

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Current standard assays to analyze lymphocyte-mediated antitumor cytotoxicity employ radioisotopic or fluorescent labels. However, such assays are not suitable for real-time analysis. Here we describe a protocol that facilitates the analysis of lymphocyte-mediated toxicity using a label-free, impedance-based real-time cell analyzer. This analyzer measures cellular electrical impedance, expressed as the cell index value, noninvasively and continuously. In contrast with label -dependent assays, this protocol simultaneously generates real-time killing curves useful for quantifying lymphocyte-mediated cytotoxicity in real time. For complete details on the use and execution of this protocol, please refer to Kanemaru et al. (2021).

引用

页数：11

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