Effect of P15INK4b expression on the cell cycle and G1 phase related regulatory genes in human melanoma cells

被引:0
|
作者
Liu, J [1 ]
Tong, YK [1 ]
Liu, HT [1 ]
Zhang, W [1 ]
Gao, P [1 ]
机构
[1] Beijing Normal Univ, Coll Life Sci, State Key Lab Cell Proliferat & Regulat Biol Educ, Beijing 100875, Peoples R China
关键词
P15(INK4b); CKI; cell cycle; G(1) phase-related gene; cell synchronization;
D O I
暂无
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Using the transfection technique, P15(INK4b) was introduced into P15(INK4b) gene deleted human melanoma A375 tells, and a cell model MLIK6 overexpressing P15(INK4b) was constructed. Comparing with the control cells MLC2, MLIK6 cells in G(t) phase increased by 11%, but those in S phase decreased by 15% by FCM. By the method of thymidine (TdR) and N2O arresting, the proportions of synchronized M phase cells of MLIK6 and MLC2 were measured and found to be 89.1% and 76.8%, respectively, and the tells in G(1) phase were 74.3% for MLIK6 and 76. 4% for MLC2. The result of H-3-TdR incorporation indicated that the transition of G(1)/S of MLIK6 cell was delayed 2 h as compared with that of MLC2 cells, and incorporation rate also decreased. The observation on expressions of some G(1)/S-related relatory regulating genes showed that in MLIK6 cells the protein level of P27(KIP1) increased with the decreasing expressions of cycllinD1, cyclinE and c-myc, especially cyclinD1 in late G(1) phase. The expression of cyclinE obviously decreased at G(1)/S transition, and c-myc was inhibited throughout all the process of G(1)-->S phase. All the results suggest that P15(INK4b) call delay G(1)/S transition of MLIK6 cells by inhibiting the cell cycle engine molecule, and by increasing the expression of Cdk inhibitor P27(KIP1) in different stages of G(1) phase.
引用
收藏
页码:40 / 45
页数:6
相关论文
共 50 条
  • [1] Effect of p15INK4b expression on the cell cycle and G1 phase related regulatory genes in human melanoma cells
    刘军
    童迎凯
    柳惠图
    张伟
    高萍
    ProgressinNaturalScience, 2001, (01) : 42 - 47
  • [2] Effect of PMA on growth and apoptosis of human melanoma cell overexpressing CKI p15INK4B
    Tong, YK
    Liu, HT
    Liu, J
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2001, 28 (02) : 198 - 202
  • [3] Expression and regulation of G1 cell-cycle inhibitors (p16INK4A , p15INK4B, p18 INK4C, p19INK4D ) in human acute myeloid leukemia and normal myeloid cells
    J Schwaller
    Th Pabst
    HP Koeffler
    G Niklaus
    P Loetscher
    MF Fey
    A Tobler
    Leukemia, 1997, 11 : 54 - 63
  • [4] Aberrant methylation and impaired expression of the p15INK4b cell cycle regulatory gene in chronic myelomonocytic leukemia (CMML)
    Tessema, M
    Länger, F
    Dingemann, J
    Ganser, A
    Kreipe, H
    Lehmann, U
    LEUKEMIA, 2003, 17 (05) : 910 - 918
  • [5] Aberrant methylation and impaired expression of the p15INK4b cell cycle regulatory gene in chronic myelomonocytic leukemia (CMML)
    M Tessema
    F Länger
    J Dingemann
    A Ganser
    H Kreipe
    U Lehmann
    Leukemia, 2003, 17 : 910 - 918
  • [6] Homozygous deletion of the p16INK4a and the p15INK4b tumour suppressor genes in a subset of human sporadic cutaneous malignant melanoma
    Wagner, SN
    Wagner, C
    Briedigkeit, L
    Goos, M
    BRITISH JOURNAL OF DERMATOLOGY, 1998, 138 (01) : 13 - 21
  • [7] Ginsenoside Rh2-mediated G1 Phase Cell Cycle Arrest in Human Breast Cancer Cells Is Caused by p15Ink4B and p27Kip1-dependent Inhibition of Cyclin-dependent Kinases
    Sunga Choi
    Tae Woong Kim
    Shivendra V. Singh
    Pharmaceutical Research, 2009, 26 : 2280 - 2288
  • [9] Myc downregulation by transforming growth factor β required for activation of the p15Ink4b G1 arrest pathway
    Warner, BJ
    Blain, SW
    Seoane, J
    Massagué, J
    MOLECULAR AND CELLULAR BIOLOGY, 1999, 19 (09) : 5913 - 5922
  • [10] Lack of Increased P15INK4B Protein Expression in Basal Cell Carcinomas
    Moad, Ahmed Ismail Hassan
    Tan, Mei Lan
    Kaur, Gurjeet
    Mabruk, Mohamed
    ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 2012, 13 (12) : 6239 - 6244