Determination of Aflatoxin B1 in Camellia Oleifera Seed by Immunoaffinity Column and High Performance Liquid Chromatography

被引：0
|
作者：
Lin, Ling [1 ,2 ]
Yang, Chun Liang [1 ,2 ]
Cha, Yu Bing [1 ,2 ]
Bin Guo, Hong [1 ,2 ]
Zeng, Ri Zhong [3 ]
机构：
[1] CATAS, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China
[2] Minist Agr Zhanjiang, Lab Qual Safety Risk Assessment Agr Prod Proc, Zhanjiang 524001, Peoples R China
[3] CATAS, Rubber Res Inst, Danzhou 571737, Peoples R China
来源：
FRONTIERS OF MANUFACTURING SCIENCE AND MEASURING TECHNOLOGY V | 2015年
关键词：
immunoaffinity column; HPLC; aflatoxin B1; camellia oleifera seed;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
A method for the determination of aflatoxin B1 in camellia oleifera seed was established by immunoaffinity column and High Performance Liquid Chromatography. After being extracted with methanol-water, the sample extract was filtered, diluted with water and through to immunoaffinity column for cleaning, methanol dissolved the residues and then determination by post column derivatization HPLC fluorescence detection. Research results show that the detection limits of aflatoxin B1 reached 0.3 mu g/kg. The recovery were more than 85 %. And the sensitivity, accuracy and precision of this method were able to meet the requirements for sample analysis.
引用
收藏
页码：764 / 769
页数：6
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