Aggregated Singletons for Automated Purification Workflow

Aggregated singletons for automated purification (ASAP) is a purification workflow that provides singleton sample purification, registration, and delivery to the materials management department as 30 mM dimethyl sulfoxide solutions for biological screening. The singleton samples submitted are aggregated in a mini-array of 10-12 samples and then are analyzed using an automated purification process. The steps in the process include pre-quality control (QC), preparative chromatography, solvent evaporation, reformat dilution and duplication, final QC, and final registration. The turnaround time from samples received to delivery to materials management is two or three business days. The final QC data are uploaded to a database and are available to chemists via electronic laboratory notebook software. The final purity, weight recovery, and registration information is available in a research database and an e-mail notification of completion is sent to the chemist. ASAP enables a high purification success rate, increases the likelihood of running mini-arrays that generate 5-10 analogs in a final step rather than one or two with the same 2-3 day turnaround time, and provides expert-level service and technology.