An ordered carbon nanotubes (CNTs) film, which was template-synthesized within the porous of an alumina template, modified glassy carbon electrode is fabricated. The electrode exhibits potent electrocatalysis for NADH oxidation in PBS (pH 6.8) with a diminution of the overpotential of more than 600 mV. The anodic peak current increases linearly with NADH concentration from 2 x 10(-5) mol/L to 1 x 10(-3) mol/L with the detection limit of about 5 x 10(-7) mol/L.