Phytoactinopolyspora halophila sp. nov., a halophilic actinomycete isolated from a saline soil

A novel halophilic actinobacterial strain, designated YIM 96934(T), was isolated from a soil sample collected from the edge of a saline lake in Xinjiang, north-west China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain YIM 96934(T) was related most closely to type strains of the genus Phytoactinopolyspora, and shared highest sequence similarity with Phytoactinopolyspora endophytica EGI 60009(T) (94.7 %), Phytoactinopolyspora alkaliphila EGI 80629(T) (94.5 %) and Phytoactinopolyspora halotolerans YIM 96448(T) (94.1 %). Optimum growth of the strain was observed at 28-37 degrees C (range 15-45 degrees C), pH 7.0-8.0 (6.0-9.0) and 5-8 % (w/v) NaCl (2-24 %). The major isoprenoid quinone was MK-9(H-4). The whole-cell sugars were glucose, galactose, mannose and rhamnose. The diagnostic diamino acid was LL-diaminopimelic acid. The polar lipid profile was found to consist of diphosphatidylglycerol, four unidentified phospholipids, three unidentified phosphoglycolipids, an unidentified aminophospholipid, two phosphatidylinositol mannosides and an unidentified polar lipid. The major fatty acids were anteiso-C-15:0, iso-C-16:0, iso-C-17:0, anteiso-C-17.0 and summed feature 4 (iso-C-17:1 I and/or anteiso-C-17:1 B). The DNA G+C content was 66.6 mol%. On the basis of phenotypic, genotypic and phylogenetic data, a novel species, Phytoactinopolyspora halophila sp. nov., is proposed. The type strain is YIM 96934(T) (=KCTC 39926(T)=CCTCC AB 2017057 (T)).