Nuclear HMGB1 promotes the phagocytic ability of macrophages

被引：15

作者：

Miao, Jifei ^{[1
]}

Ye, Sen ^{[1
]}

Lan, Jiao ^{[2
]}

Ye, Peng ^{[1
]}

Wen, Quan ^{[3
]}

Mei, Liyan ^{[1
]}

Liu, Xia ^{[4
]}

Lin, Junli ^{[1
]}

Zhou, Xiaojing ^{[1
]}

Du, Shaohui ^{[5
]}

Liu, Xiaoyi ^{[1
]}

Li, Hui ^{[1
]}

机构：

[1] Guangzhou Univ Chinese Med, Res Ctr Integrat Med, Sch Basic Med Sci, Guangzhou 510006, Guangdong, Peoples R China

[2] Guangzhou Univ Chinese Med, Clin Med Acupuncture Moxibust & Rehabil, Guangzhou 510006, Guangdong, Peoples R China

[3] Jinan Univ, Guangdong Hong Kong Macau Inst CNS Regenerat GHMI, Guangzhou 510006, Guangdong, Peoples R China

[4] Guizhou Univ Chinese Med, Sch Basic Med Sci, Guiyang 550025, Guizhou, Peoples R China

[5] Guangzhou Univ Chinese Med, Shenzhen Affiliated Hosp, Shenzhen 518000, Peoples R China

来源：

EXPERIMENTAL CELL RESEARCH | 2020年 / 393卷 / 01期

基金：

中国国家自然科学基金;

关键词：

HMGB1; Phagocytosis; P53; FAK; Macrophage; FOCAL ADHESION KINASE; IMMUNOSUPPRESSION; RECOGNITION; EXPRESSION; BINDING; SEPSIS; DOMAIN; CD11B; FAK;

D O I：

10.1016/j.yexcr.2020.112037

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Phagocytosis is a basic immune response to the invasion of pathogens. High mobility group protein B1 (HMGB1) is a DNA chaperone that is associated with phagocytosis. However, its influence on phagocytosis is debated. In the present study, HMGB1-mutant, HMGB1-overexpressing and HMGB1-silenced RAW264.7 cells were constructed. In addition, HMGB1 conditional knockout mice were constructed to determine the influence of HMGB1 on phagocytosis. Lipopolysaccharide (LPS) was used to stimulate the translocation of HMGB1 from the nucleus to the cytoplasm. Zymosan particles were used to test the phagocytic function of the macrophages. Our results showed that the accumulation of HMGB1 in the nucleus enhances the phagocytic function of the macrophages. By interacting with P53, nuclear HMGB1 may remain in the nucleus and decrease the negative influence of P53 on the phosphorylation of focal adhesion kinase (FAK). The increase in phosphorylated FAK promotes the formation of pseudopods and enhances the phagocytic ability of macrophages.

引用

页数：11

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