Osteogenic capacity of human BM-MSCs, AT-MSCs and their co-cultures using HUVECs in FBS and PL supplemented media

被引：19

作者：

Ma, Jinling ^{[1
,2
]}

van den Beucken, Jeroen J. J. P. ^{[1
]}

Both, Sanne K. ^{[1
]}

Prins, Henk-Jan ^{[3
,4
]}

Helder, Marco N. ^{[5
]}

Yang, Fang ^{[1
]}

Jansen, John A. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Med Ctr, Dept Biomat, NL-6525 EX Nijmegen, Netherlands

[2] Capital Med Univ, Beijing Stomatol Hosp, Dept Oral & Maxillofacial Surg, Beijing, Peoples R China

[3] Acad Ctr Dent Amsterdam ACTA, Dept Oral Cell Biol, Amsterdam, Netherlands

[4] Vrije Univ Amsterdam, Dept Oral & Maxillofacial Surg, Med Ctr, Acad Ctr Dent Amsterdam, Amsterdam, Netherlands

[5] Vrije Univ Amsterdam, Dept Orthoped Surg, Med Ctr, Amsterdam, Netherlands

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2015年 / 9卷 / 07期

关键词：

platelet lysate; fetal bovine serum; bone tissue engineering; stem cells; co-culture; osteogenesis; MESENCHYMAL STEM-CELLS; HUMAN PLATELET LYSATE; MARROW STROMAL CELLS; FETAL BOVINE SERUM; BONE-MARROW; ADIPOSE-TISSUE; PROGENITOR CELLS; ENDOTHELIAL-CELLS; SCALE EXPANSION; ANIMAL SERUM;

D O I：

10.1002/term.1704

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Human bone marrow-derived mesenchymal stem cells (BM-MSCs) and human adipose tissue-derived mesenchymal stem cells (AT-MSCs) are the most frequently used stem cells in tissue engineering. Due to major clinical demands, it is necessary to find an optimally safe and efficient way for large-scale expansion of these cells. Considering the nutritional source in the culture medium and method, this study aimed to analyze the effects of FBS- and PL-supplemented media on osteogenesis in stem cell mono- and co-cultures with human umbilical vein endothelial cells (HUVECs). Results showed that cell metabolic activity and proliferation increased in PL- compared to FBS-supplemented media in mono- and co-cultures for both BM-MSCs and AT-MSCs. In addition, calcium deposition was cell type dependent and decreased for BM-MSCs but increased for AT-MSCs in PL-supplemented medium in both mono- and co-cultures. Based on the effects of co-cultures, BM-MSCs/HUVECs enhanced osteogenesis compared to BM-MSCs monocultures in both FBS- and PL-supplemented media whereas AT-MSCs/HUVECs showed similar results compared to AT-MSCs monocultures. Copyright (c) 2013 John Wiley & Sons, Ltd.

引用

页码：779 / 788

页数：10

共 2 条

[1] In vitro and in vivo angiogenic capacity of BM-MSCs/HUVECs and AT-MSCs/HUVECs cocultures
Ma, Jinling
Yang, Fang
Both, Sanne K.
Prins, Henk-Jan
Helder, Marco N.
Pan, Juli
Cui, Fu-Zhai
Jansen, John A.
van den Beucken, Jeroen J. J. P.
[J]. BIOFABRICATION, 2014, 6 (01)
[2] Comparing the immunomodulatory properties of equine BM-MSCs culture expanded in autologous platelet lysate, pooled platelet lysate, equine serum and fetal bovine serum supplemented culture media
Even, Kayla M.
Gaesser, Angela M.
Ciamillo, Sarah A.
Linardi, Renata L.
Ortved, Kyla F.
[J]. FRONTIERS IN VETERINARY SCIENCE, 2022, 9

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