Dissecting intercellular signaling with mass spectrometry-based proteomics

被引:11
|
作者
Swietlik, Jonathan J. [1 ]
Sinha, Ankit [1 ,2 ]
Meissner, Felix [1 ]
机构
[1] Max Planck Inst Biochem, Expt Syst Immunol Lab, Martinsried, Germany
[2] Tech Univ Munich, Klinikum Rechts Isar, Inst Translat Canc Res & Expt Canc Therapy, Munich, Germany
关键词
Proteomics; Mass spectrometry; Intercellular; Signaling; Cell non-autonomous; Posttranslational modification; PTM; Sender; Receiver; Messenger; Interactions; Heterocellular; Paracrine; Autocrine; Receptor; Ligand; Interaction; LIVING CELLS; IDENTIFICATION; QUANTIFICATION; PROTEINS; LOCALIZATION; EXPLORATION; ENRICHMENT; SECRETOME; REVEALS; NETWORK;
D O I
10.1016/j.ceb.2019.12.002
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Physiological functions depend on a coordinated interplay of numerous different cell types. Proteins serve as major signaling molecules between cells; however, their comprehensive investigation in physiologically relevant settings has remained challenging. Mass spectrometry (MS)-based shotgun proteomics is emerging as a powerful technology for the systematic analysis of protein-mediated intercellular signaling and regulated post-translational modifications. Here, we discuss recent advancements in cell biological, chemical, and biochemical MS-based approaches for the profiling of cellular messengers released by sending cells, receptors expressed on the cell surface, and their interactions. We highlight methods tailored toward the mapping of dynamic signal transduction mechanisms at cellular interfaces and approaches to dissect communication cell specifically in heterocellular systems. Thereby, MS-based proteomics contributes a unique systems biology perspective for the identification of intercellular signaling pathways deregulated in disease.
引用
收藏
页码:20 / 30
页数:11
相关论文
共 50 条
  • [1] Dissecting plasmodesmata molecular composition by mass spectrometry-based proteomics
    Salmon, Magali S.
    Bayer, Emmanuelle M. F.
    [J]. FRONTIERS IN PLANT SCIENCE, 2013, 3
  • [2] Mass spectrometry-based protein analysis and signaling proteomics
    Taniguchi, H
    [J]. SEIKAGAKU, 2004, 76 (10): : 1289 - 1295
  • [3] Mass spectrometry-based proteomics
    Ruedi Aebersold
    Matthias Mann
    [J]. Nature, 2003, 422 : 198 - 207
  • [4] Mass spectrometry-based proteomics
    Aebersold, R
    Mann, M
    [J]. NATURE, 2003, 422 (6928) : 198 - 207
  • [5] Mass spectrometry-based proteomics
    Hood, BL
    Veenstra, TD
    Conrads, TP
    [J]. ADVANCES IN FERTILITY AND REPRODUCTIVE MEDICINE, 2004, 1266 : 375 - 380
  • [6] Mass spectrometry-based quantitative proteomics
    Heck, AJR
    Krijgsveld, J
    [J]. EXPERT REVIEW OF PROTEOMICS, 2004, 1 (03) : 317 - 326
  • [7] An Introduction to Mass Spectrometry-Based Proteomics
    Shuken, Steven R.
    [J]. JOURNAL OF PROTEOME RESEARCH, 2023, 22 (07) : 2151 - 2171
  • [8] Computational Mass Spectrometry-Based Proteomics
    Kall, Lukas
    Vitek, Olga
    [J]. PLOS COMPUTATIONAL BIOLOGY, 2011, 7 (12)
  • [9] Mass spectrometry-based targeted proteomics
    Doerr, Allison
    [J]. NATURE METHODS, 2013, 10 (01) : 23 - 23
  • [10] ADVANCES IN MASS SPECTROMETRY-BASED PROTEOMICS
    Anderson, Rby Damon
    [J]. Lab Manager, 2023, 18 (02): : 46 - 47