Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy

被引：18
|
作者：
Rebrosova, Katarina [1 ,2 ]
Siler, Martin [3 ]
Samek, Ota [3 ]
Ruzicka, Filip [1 ,2 ]
Bernatova, Silvie [3 ]
Jezek, Jan [3 ]
Zemanek, Pavel [3 ]
Hola, Veronika [1 ,2 ]
机构：
[1] Fac Med, Dept Microbiol, Pekarska 53, Brno 65691, Czech Republic
[2] St Annes Fac Hosp, Pekarska 53, Brno 65691, Czech Republic
[3] ASCR, Inst Sci Instruments, Kralovopolska 147, Brno 61264, Czech Republic
来源：
FUTURE MICROBIOLOGY | 2017年 / 12卷 / 10期
关键词：
Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis; Staphyococcus aureus; COAGULASE-NEGATIVE STAPHYLOCOCCI; BIOFILM FORMATION; IDENTIFICATION; BACTERIA; PATHOGENS; CELLS; MICROSPECTROSCOPY; SPECTRA;
D O I：
10.2217/fmb-2016-0224
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique. Materials & methods: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. Discussion & conclusion: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.
引用
收藏
页码：881 / 890
页数：10
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