Structure determination and optimization of a cysteine-less intein with NMR spectroscopy and molecular dynamics simulations

被引:0
|
作者
Sievers, Elanor E. [1 ]
Berkeley, Raymond F. [1 ]
Debelouchina, Galia T. [1 ]
机构
[1] Univ Calif San Diego, Chem & Biochem, La Jolla, CA 92093 USA
关键词
D O I
暂无
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
879-Pos
引用
收藏
页码:181A / 181A
页数:1
相关论文
共 50 条
  • [1] A mesophilic cysteine-less split intein for protein trans-splicing applications under oxidizing conditions
    Bhagawati, Maniraj
    Terhorst, Tobias M. E.
    Fuesser, Friederike
    Hoffmann, Simon
    Pasch, Tim
    Pietrokovski, Shmuel
    Mootz, Henning D.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (44) : 22164 - 22172
  • [2] Optimization of designed armadillo repeat proteins by molecular dynamics simulations and NMR spectroscopy
    Alfarano, Pietro
    Varadamsetty, Gautham
    Ewald, Christina
    Parmeggiani, Fabio
    Pellarin, Riccardo
    Zerbe, Oliver
    Plueckthun, Andreas
    Caflisch, Amedeo
    PROTEIN SCIENCE, 2012, 21 (09) : 1298 - 1314
  • [3] Structural and biochemical analysis of a novel atypically split intein reveals a conserved histidine specific to cysteine-less inteins
    Pasch, Tim
    Schroeder, Alexander
    Kattelmann, Sabrina
    Eisenstein, Miriam
    Pietrokovski, Shmuel
    Kuemmel, Daniel
    Mootz, Henning D.
    CHEMICAL SCIENCE, 2023, 14 (19) : 5204 - 5213
  • [4] X-ray structure of a cysteine-less mutant galectin-1
    Yoshida, Hiromi
    Abe, Akemi
    Nishi, Nozomu
    Nakamura, Takanori
    Kamitori, Shigehiro
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2008, 64 : C311 - C311
  • [5] Understanding the Catalytic Mechanism of Spl DnaX intein-mediated Protein Splicing guided by Solution NMR Spectroscopy and Molecular Dynamics Simulations
    Boral, Soumendu
    Kushwaha, Tushar
    Inampudi, Krishna K.
    De, Soumya
    PROTEIN SCIENCE, 2021, 30 : 53 - 54
  • [6] Structure and Dynamics of Mycobacterium tuberculosis Truncated Hemoglobin N: Insights from NMR Spectroscopy and Molecular Dynamics Simulations
    Savard, Pierre-Yves
    Daigle, Richard
    Morin, Sebastien
    Sebilo, Anne
    Meindre, Fanny
    Laguee, Patrick
    Guertin, Michel
    Gagne, Stephane M.
    BIOCHEMISTRY, 2011, 50 (51) : 11121 - 11130
  • [7] MOLECULAR-STRUCTURE DETERMINATION BY NMR-SPECTROSCOPY
    BUCKINGHAM, AD
    PURE AND APPLIED CHEMISTRY, 1974, 40 (1-2) : 1 - 11
  • [8] Conformational analysis of a tetrasaccharide based on NMR spectroscopy and molecular dynamics simulations
    Landersjö, C
    Jansson, JLM
    Maliniak, A
    Widmalm, G
    JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (36): : 17320 - 17326
  • [9] SOLUTION STRUCTURE OF THE LEWIS X OLIGOSACCHARIDE DETERMINED BY NMR-SPECTROSCOPY AND MOLECULAR-DYNAMICS SIMULATIONS
    MILLER, KE
    MUKHOPADHYAY, C
    CAGAS, P
    BUSH, CA
    BIOCHEMISTRY, 1992, 31 (29) : 6703 - 6709
  • [10] Lipid Bilayer Structure and Dynamics Studied with Molecular Dynamics Simulations and NMR Measurements
    Ferreira, Tiago
    Topgaard, Daniel
    Ollila, Samuli O. H.
    BIOPHYSICAL JOURNAL, 2014, 106 (02) : 41A - 41A