Application of benzo[a]phenoxazinium chlorides in antimicrobial photodynamic therapy of Candida albicans biofilms

被引:30
|
作者
Lopes, Marisa [1 ]
Alves, Carlos Tiago [1 ]
Rama Raju, B. [2 ,3 ]
Goncalves, M. Sameiro T. [2 ]
Coutinho, Paulo J. G. [3 ]
Henriques, Mariana [1 ]
Belo, Isabel [1 ]
机构
[1] Univ Minho, Ctr Biol Engn, P-4710057 Braga, Portugal
[2] Univ Minho, Ctr Chem, P-4710057 Braga, Portugal
[3] Univ Minho, Ctr Phys, P-4710057 Braga, Portugal
关键词
Candida albicans; Biofilms; Antimicrobial photodynamic therapy; Benzo[a]phenoxazines; PORPHINE TETRA TOSYLATE; KERATINOCYTES IN-VITRO; METHYLENE-BLUE; INACTIVATION; INFECTIONS; RESISTANCE; AGENTS; PHOTOSENSITIZATION; SUSCEPTIBILITY; PATHOGENICITY;
D O I
10.1016/j.jphotobiol.2014.09.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The use of Antimicrobial Photodynamic Therapy (APDT) as a new approach to treat localized Candida infections is an emerging and promising field nowadays. The aim of this study was to verify the efficacy of photodynamic therapy using two new benzo[a]phenoxazinium photosensitizers against Candida albicans biofilms: N-(5-(3-hydroxypropylamino)-10-methyl-9H-benzo[a]phenoxazin-9-ylidene)ethanaminium chloride (FSc) and N-(5-(11-hydroxyundecylamino)-10-methyl-9H-benzo[a]phenoxazin-9-ylidene)ethanaminium chloride (FSd). The photodynamic activity of dyes against C albicans biofilms was evaluated by incubating biofilms with dyes in the range of 100-300 mu M for 3 or 18 h followed by illumination at 12 or 36 J cm(-2), using a xenon arc lamp (600 +/- 2 nm). A total photoinactivation of C albicans biofilm cells was achieved using 300 mu M of FSc with 18 h of incubation, followed by illumination at 36 J cm(-2). Contrarily, FSd had insignificant effect on biofilms inactivation by APDT. The higher uptake of FSc than FSd dye by biofilms during the dark incubation may explain the greater photodynamic effectiveness achieved with FSc. The results obtained stresses out the FSc-mediated APDT potential use to treat C albicans infections. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:93 / 99
页数:7
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