Synthesis of chondroitin sulfate magnesium for osteoarthritis treatment

被引：39
|
作者：
Li Sijing [1 ,2 ]
Ma Fenbo [2 ]
Pang Xiangchao [2 ,5 ]
Tang Bin [2 ,3 ,4 ]
Lin Lijun [1 ]
机构：
[1] Southern Med Univ, Zhujiang Hosp, Dept Orthoped, Guangzhou, Guangdong, Peoples R China
[2] Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Guangdong, Peoples R China
[3] Guangdong Prov Key Lab Cell Microenvironm & Dis R, Guangzhou, Guangdong, Peoples R China
[4] Shenzhen Key Lab Cell Microenvironm, Shenzhen, Peoples R China
[5] Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha, Hunan, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2019年 / 212卷
关键词：
Chondroitin sulfate; Magnesium; Anti-inflammatory; Apoptosis; NITRIC-OXIDE SYNTHASE; CHONDROCYTE APOPTOSIS; SELECTIVE-INHIBITION; KNEE OSTEOARTHRITIS; IN-VIVO; KAPPA-B; PROGRESSION; DEFICIENCY; EFFICACY; SAFETY;
D O I：
10.1016/j.carbpol.2019.02.061
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Magnesium chondroitin sulfate (MgCS) has been fabricated and characterized in this study. We investigated its morphology, composition as well as structure. The results verify that the sodium of sodium chondroitin sulfate (CS) is successfully replaced by magnesium and formed a polysaccharide-metal ion complex. To evaluate the clinical potential of MgCS, cell proliferation and apoptosis test were conducted. The results reveal that MgCS could effectively increase the proliferation and decrease the apoptosis of osteoarthritis (OA) chondrocytes. Moreover, real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to evaluate the gene expression level. RT-qPCR analysis suggests that MgCS could significantly increase the expression of COLII and decrease the expression of IL-1 beta and iNOS in OA chondrocytes. Furthermore, significant upregulation of Bcl-2 mRNA expression and downregulation of the expression of apoptosis related gene p53 were observed. Thus, it is indicated that MgCS should have great potentials in OA treatment.
引用
收藏
页码：387 / 394
页数：8
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