An Alternative Method for Cultivation of Lawsonia intracellularis

被引:24
|
作者
Vannucci, Fabio A. [1 ]
Wattanaphansak, Suphot [1 ]
Gebhart, Connie J. [1 ]
机构
[1] Univ Minnesota, Coll Vet Med, St Paul, MN 55108 USA
关键词
PORCINE PROLIFERATIVE ENTEROPATHY; ASSAY;
D O I
10.1128/JCM.05976-11
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An alternative method for the cultivation of Lawsonia intracellularis, an obligate intracellular bacterium and the causative agent of proliferative enteropathy, was developed using an Original Space Bag inflated with a mixture of gas containing 10% hydrogen, 10% carbon dioxide, and 80% nitrogen. The flexibility of this protocol allows the testing of various environmental conditions for static cultivation of this bacterium and the development of diagnostic techniques.
引用
收藏
页码:1070 / 1072
页数:3
相关论文
共 50 条
  • [1] In vitro cultivation and immunostaining of Lawsonia intracellularis strains
    Szczotka-Bochniarz, Anna
    Podgorska, Katarzyna
    Nowak, Agnieszka
    Pejsak, Zygmunt
    [J]. BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY, 2013, 57 (03) : 319 - 322
  • [2] Isolation and In Vitro cultivation of Lawsonia intracellularis from China
    Xiao, Ning
    Li, Jiannan
    Li, Minxue
    Zhou, Hong
    Lin, Huixing
    Fan, Hongjie
    [J]. VETERINARY MICROBIOLOGY, 2022, 270
  • [3] Cultivation and characterization of Lawsonia intracellularis isolated from rabbit and pig
    Watarai, Masahisa
    Yoshiya, Masahiro
    Sato, Akane
    Furuoka, Hidefumi
    [J]. JOURNAL OF VETERINARY MEDICAL SCIENCE, 2008, 70 (07): : 731 - 733
  • [4] Immunohistochemical method for identification of Lawsonia intracellularis infection in pigs
    Surpat , Anca Sofiana
    Herman, Viorel
    Marincu, Iosif
    Mederle, Narcisa
    Mederle, Ovidiu Alexandru
    [J]. JOURNAL OF BIOTECHNOLOGY, 2015, 208 : S100 - S100
  • [5] A slide-SAB method for the detection of the antibody of Lawsonia intracellularis
    Sueyoshi, M.
    Tsurita, M.
    Sasaki, Y.
    Uemura, R.
    [J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2014, 21 : 191 - 191
  • [6] Measurement of the viability of Lawsonia intracellularis
    Wattanaphansak, S
    Gebhart, C
    Olin, M
    Deen, J
    [J]. CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 2005, 69 (04): : 265 - 271
  • [7] Lawsonia intracellularis proliferative enteropathy in a foal
    Feary, D. J.
    Gebhart, C. J.
    Pusterla, N.
    [J]. SCHWEIZER ARCHIV FUR TIERHEILKUNDE, 2007, 149 (03): : 129 - 133
  • [8] Foals wanted: Lawsonia intracellularis in horses
    van Oldruitenborgh-Oosterbaan, Marianne Sloet
    [J]. TIJDSCHRIFT VOOR DIERGENEESKUNDE, 2008, 133 (17) : 720 - 720
  • [9] Lawsonia intracellularis and swine enteric disease
    Moore, GM
    Shryock, TR
    [J]. COMPENDIUM ON CONTINUING EDUCATION FOR THE PRACTICING VETERINARIAN, 1996, 18 (01): : S11 - +
  • [10] Lawsonia intracellularis and Equine Proliferative Enteropathy
    Page, Allen E.
    Slovis, Nathan M.
    Horohov, David W.
    [J]. VETERINARY CLINICS OF NORTH AMERICA-EQUINE PRACTICE, 2014, 30 (03) : 641 - +