A co-expression vector for baculovirus-mediated protein expression in mammalian cells

被引：4
|
作者：
Guo, Wenjun [1 ]
Wang, Mengmeng [1 ,2 ,3 ]
Chen, Lei [1 ,2 ,3 ]
机构：
[1] Peking Univ, Inst Mol Med, Coll Future Technol, State Key Lab Membrane Biol,Beijing Key Lab Cardi, Beijing 100871, Peoples R China
[2] Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
[3] Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2022年 / 594卷
基金：
中国国家自然科学基金;
关键词：
BacMam; Baculovirus; Membrane protein; TRPC; K-ATP; EFFICIENT GENE-TRANSFER; SULFONYLUREA RECEPTOR; TRANSDUCTION; INSECT;
D O I：
10.1016/j.bbrc.2022.01.056
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
BacMam system utilizes baculovirus to deliver exogenous genes into mammalian cells and is extensively used for recombinant production of eukaryotic proteins. Here, we described the development of a BacMam vector (pBMCL1), which allows convenient tracing of virus production, provides higher infection efficiency towards mammalian cells, minimizes unwanted transcription of toxic genes in insect cells, and provides the capability for co-expression of multiple proteins via a single virus. We demonstrate the successful application of the pBMCL1 vector for the expression of homo-tetrameric human TRPC3 channel and hetero-octameric KATP channel. (C) 2022 Elsevier Inc. All rights reserved.
引用
收藏
页码：69 / 73
页数：5
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