Dietary supplementation withBacillus subtilisDSM 32315 alters the intestinal microbiota and metabolites in weaned piglets

Aim The study was conducted to investigate the effects of dietaryBacillus subtilis(BS) DSM 32315 on the intestinal microbiota composition and metabolites of weaned pigs. Methods and Results Sixty-four piglets were allocated to two groups (control andBS), each group including eight replicates with four piglets. DietaryBSDSM 32315 increased (P < 0 center dot 05) the abundances of jejunalLeucobacterandCupriavidus, ilealThermus,CoprococcusandBifidobacterium, as well as colonicSucciniclasticum; and increased the concentrations of ileal straight-chain fatty acids, colonic propionate, branched-chain fatty acids (BCFAs), and tyramine, but decreased (P .05) the colonic indole concentration. The ileal and colonic microbial community structure tended to cluster into two groups. LEfSe analysis identified five microbial biomarkers in jejunum and eight biomarkers in ileum in theBSgroup, and three biomarkers in colon in the control group. The ilealBifidobacteriumabundance was positively correlated (P < 0 center dot 05) with isovalerate concentration, while the colonicActinobacteriaandLactobacillusabundances were negatively correlated (P < 0 center dot 05) with indole concentration. Conclusion These findings suggest that dietary supplementation withBSDSM 32315 could alter the diversity, composition, and metabolites of intestinal microbiota in weaned piglets. Significance and Impact of the Study Weaned piglets are often accompanied with impaired gastrointestinal tract and intestinal disorder affecting their growth. This study demonstrated that dietaryBSDSM 32315 presented a beneficial role in gut health via regulating intestinal microbiota composition and metabolites.