High Throughput Miniaturization of Artificial Cells

被引:8
|
作者
Mobed-Miremadi, Maryam [1 ]
Acks, Erik [1 ]
Polsaward, Sutthipong [1 ]
Chen, Ding [1 ]
机构
[1] San Jose State Univ, Dept Gen Engn, San Jose, CA 95192 USA
关键词
Bio-printing; miniaturization; inkjet; dimensionless analysis; high throughput; artificial cells; FABRICATION;
D O I
10.3109/10731199.2011.574637
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this study, inkjet bio-printing has been used to produce miniaturized alginate microcapsules. A parametric study using subsequent Taguchi L-18 (3(1) X 2(7)) and L-16 (4(5)) designs was performed to elucidate the effect of inkjet parameters on microcapsule size. A 120-minute pilot run using the optimal waveform parameters and 0.5% alginate ink yielded a throughput of 1.8 X 10(6) microcapsules/hr, averaging 40 mu m in diameter. Real-time stable jetting conditions were confirmed visually by the generation of a single droplet with a straight trajectory and non-fluctuating Ohnesorge numbers. The rate of stirring of the cross-linking CaCl2 solution determined scaffold vs. single vesicle formation.
引用
收藏
页码:310 / 316
页数:7
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