Identifying Cell-Type Specific Genes and Expression Rules Based on Single-Cell Transcriptomic Atlas Data

被引：8
|
作者：
Yuan, Fei [1 ,2 ]
Pan, Xiao Yong [3 ,4 ]
Zeng, Tao [5 ]
Zhang, Yu-Hang [6 ]
Chen, Lei [7 ,8 ]
Gan, Zijun [6 ]
Huangs, Tao [6 ]
Cai, Yu-Dong [1 ]
机构：
[1] Shanghai Univ, Sch Life Sci, Shanghai, Peoples R China
[2] Binzhou Med Univ Hosp, Dept Sci & Technol, Binzhou, Peoples R China
[3] Shanghai Jiao Tong Univ, Inst Image Proc & Pattern Recognit, Shanghai, Peoples R China
[4] Minist Educ China, Key Lab Syst Control & Informat Proc, Shanghai, Peoples R China
[5] Chinese Acad Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, Shanghai, Peoples R China
[6] Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Shanghai Inst Biol Sci, Shanghai, Peoples R China
[7] Shanghai Maritime Univ, Coll Informat Engn, Shanghai, Peoples R China
[8] East China Normal Univ, Shanghai Key Lab Pure Math & Math Practice, Shanghai, Peoples R China
来源：
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2020年 / 8卷 / 08期
基金：
国家重点研发计划; 中国国家自然科学基金; 上海市自然科学基金;
关键词：
cell type; expression rule; single-cell transcriptomics; tissue development; multi-class classification; FEATURE-SELECTION; NORMAL-TISSUES; PITX1; LEGUMAIN; RECEPTOR; PERP; DIFFERENTIATION; IDENTIFICATION; ACTIVATION; GALECTIN-7;
D O I：
10.3389/fbioe.2020.00350
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Single-cell sequencing technologies have emerged to address new and longstanding biological and biomedical questions. Previous studies focused on the analysis of bulk tissue samples composed of millions of cells. However, the genomes within the cells of an individual multicellular organism are not always the same. In this study, we aimed to identify the crucial and characteristically expressed genes that may play functional roles in tissue development and organogenesis, by analyzing a single-cell transcriptomic atlas of mice. We identified the most relevant gene features and decision rules classifying 18 cell categories, providing a list of genes that may perform important functions in the process of tissue development because of their tissue-specific expression patterns. These genes may serve as biomarkers to identify the origin of unknown cell subgroups so as to recognize specific cell stages/states during the dynamic process, and also be applied as potential therapy targets for developmental disorders.
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页数：11
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