Efficacy of anti-CD20 chimeric Fab′ fragment on proliferation of B lymphoma cells

被引:4
|
作者
Lai, ZZ
Xiong, DS
Fan, DM
Xu, YF
Liu, HZ
Peng, H
Zhu, ZP
Yang, CZ [1 ]
机构
[1] Chinese Acad Med Sci, Inst Hematol, Natl Lab Expt Hematol, Tianjin 300020, Peoples R China
[2] Beijing Union Med Coll, Tianjin 300020, Peoples R China
来源
CHINESE SCIENCE BULLETIN | 2001年 / 46卷 / 14期
关键词
monoclonal antibody; chimeric Fab '; B lymphoma; CD20;
D O I
10.1007/BF02900598
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The variable domain of heavy chain (V-H) and light chain (V-L) genes of anti-CD20 monoclonal antibody HI47 were cloned from anti-CD20 ScFv expression vector pCANBTEcd20 by PCR and ligated into vector pYZF to construct chimeric anti-CD20 Fab' fragment expression vector pYZFcd20. Chimeric anti-CD20 Fab' fragment was expressed in E. coli 16C9 and purified by protein G affinity chromatography. Competitive inhibition assay showed that anti-CD20 Fab' fragment inhibited binding of HI47 to CD20 on the surface of Daudi cells. Results from MTT assay indicated that chimeric anti-CD20 Fab' fragment inhibited the proliferation of Daudi cells, IC50 = 69 mug/mL. Affinity of chimeric anti-CD20 Fab' fragment was determined, K-a was about 8.9x10(8) (mol/L)(-1).
引用
收藏
页码:1182 / 1186
页数:5
相关论文
共 50 条
  • [1] Efficacy of anti-CD20 chimeric Fab' fragment on proliferation of B lymphoma cells
    LAI Zengzu
    [J]. Science Bulletin, 2001, (14) : 1182 - 1186
  • [2] Homology modeling of anti-CD20 chimeric Fab' fragment
    Peng, Hui
    Yang, Chunzheng
    Fan, Dongmei
    Zheng, Mengjie
    Lai, Zengzu
    Xiong, Dongsheng
    Zhu, Zhenping
    [J]. Gaojishu Tongxin/High Technology Letters, 2001, 11 (12):
  • [3] BLOCKING B-CELLS (ANTI-CD20/ANTI-CD20)
    Isenberg, David A.
    [J]. RHEUMATOLOGY, 2015, 54 : 1 - 1
  • [4] Mechanism of action on B cell lymphoma by chimeric anti-CD20 monoclonal antibody
    T Oyama
    Y Kagami
    M Seto
    Y Morishima
    [J]. Leukemia, 2001, 15 : 1667 - 1667
  • [5] Mechanism of action on B cell lymphoma by chimeric anti-CD20 monoclonal antibody
    Oyama, T
    Kagami, Y
    Seto, M
    Morishima, Y
    [J]. LEUKEMIA, 2001, 15 (10) : 1667 - 1667
  • [6] Inhibition of human B-cell lymphoma by an anti-CD20 antibody and its chimeric F(ab′)2 fragment via induction of apoptosis
    Liu, YX
    Zheng, MJ
    Lai, ZZ
    Xiong, DS
    Fan, DM
    Xu, YF
    Peng, H
    Shao, XF
    Xu, YS
    Yang, M
    Wang, JH
    Liu, HZ
    Xie, Y
    Yang, CZ
    Zhu, ZP
    [J]. CANCER LETTERS, 2004, 205 (02) : 143 - 153
  • [7] Effects of ultrasound on apoptosis induced by anti-CD20 antibody in CD20-positive B lymphoma cells
    Danno, Daisuke
    Kanno, Masatoshi
    Fujimoto, Shinichi
    Feril, Loreto B., Jr.
    Kondo, Takashi
    Nakamura, Shinobu
    [J]. ULTRASONICS SONOCHEMISTRY, 2008, 15 (04) : 463 - 471
  • [8] Anti-Tumor Effects of Anti-CD20 Chimeric Antigen Receptor Therapy in Canine B Cell Lymphoma Patients
    Panjwani, M. Kazim
    Gnanandarajah, Josephine S.
    MaloneyHuss, Martha A.
    Mason, Nicola J.
    [J]. MOLECULAR THERAPY, 2016, 24 : S296 - S296
  • [9] Anti-CD20 (rituximab) in follicular lymphoma
    McLaughlin, P
    [J]. EUROPEAN JOURNAL OF CANCER, 1999, 35 : S51 - S51
  • [10] MODULATION OF CD20 EXPRESSION ON B CELLS BY ANTI-CD20 ANTIBODIES
    Bellintani, Elaine C.
    de Marco, Renato
    Fantini, Renata
    Valim, Tiago
    Macedo, Denise
    Miyamoto, Yuriko
    Temin, Julia
    Gerbase-DeLima, Maria
    [J]. HUMAN IMMUNOLOGY, 2016, 77 : 138 - 138