Lacidipine attenuates TNF-α-induced cardiomyocyte apoptosis

被引：14

作者：

Wang, Qiu-Lin ^{[1
]}

Zhao, Lei ^{[2
]}

Feng, Na ^{[2
]}

Zhou, Peng ^{[1
]}

Wu, Qi ^{[1
]}

Fan, Rong ^{[2
]}

Li, Juan ^{[2
]}

Zhang, Shu-Miao ^{[2
]}

Wang, Yue-Min ^{[2
]}

Xu, Xue-Zeng ^{[3
]}

Yu, Shi-Qiang ^{[3
]}

Yi, Ding-Hua ^{[3
]}

Pei, Jian-Ming ^{[2
,3
]}

机构：

[1] Chengdu Med Coll, Dept Cardiol, Chengdu, Sichuan Provinc, Peoples R China

[2] Fourth Mil Med Univ, Dept Physiol, Natl Key Discipline Cell Biol, Xian 710032, Shaanxi Provinc, Peoples R China

[3] Fourth Mil Med Univ, Xijing Hosp, Dept Cardiosurg, Xian 710032, Shaanxi Provinc, Peoples R China

来源：

CYTOKINE | 2015年 / 71卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Lacidipine; Apoptosis; Caspase-12; TNF-alpha; SIGNALING PATHWAYS; REPERFUSION INJURY; BLOOD-PRESSURE; CELL-DEATH; CALRETICULIN; HEART; MECHANISMS; EXPRESSION; CASPASE-8; PROTEINS;

D O I：

10.1016/j.cyto.2014.08.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was designed to investigate whether lacidipine elicited a protective role on cardiomyocyte against apoptosis induced by TNF-alpha. Neonatal rat cardiomyocytes were randomly assigned into different groups. TUNEL staining was utilized to detect apoptosis, and caspase-3 and caspse-12 were determined. To explore the underlying mechanism, Z-ATAD-FMK (a selective caspase-12 inhibitor) was used to identify the key molecule involved. TNF-alpha increased caspase-3 expression, which was mediated by increased caspase-12 expression. In the meantime, apoptosis was significantly induced by TNF-alpha Lacidipine lowered caspase-12 and caspase-3 expression, and cardiomyocyte apoptosis induced by TNF-alpha. The results suggest that lacidipine attenuates TNF-alpha-induced apoptosis via inhibition of caspase-12 and caspase-3 successively. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：60 / 65

页数：6

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