Landing microextraction sediment phase onto surface enhanced Raman scattering to enhance sensitivity and selectivity for chromium speciation in food and environmental samples

The current study explores the first full mode liquid microextraction technique coupled with surface-enhanced Raman spectroscopy (SERS), and has been successfully applied for chromium speciation in food and environmental matrices. Herein, chromium as chlorochromate anion [CrO3Cl](-) and the cationic rhodamine 6G [RG](+) dye has been extracted in organic phase as a complex ion associate [RG+.CrO3Cl-.nS](org) at pH = 1.0. Afterwards, the extracted phase was deposited on the surface of the nano-flower shaped silver nanoparticles substrate and the SERS response was monitored against the reagent blank at 1505 cm(-1). Substrate characterizations, reaction mechanism assignment, stoichiometry, speciation, analytical applications, selectivity and validation were performed. The analytical procedure exhibits a detection limit of 0.03 mu g L-1 under the optimized experimental conditions. The accuracy of the proposed strategy was validated by inductively coupled plasma optical emission spectrometry method using student's t-and F tests at 95% confidence.