Identification of self-incompatibility genotypes of apricot (Prunus armeniaca L.) by S-allele-specific PCR analysis

被引：24

作者：

Qi, J

Gai, SP

Zhang, JX

Gu, MR

Shu, HR ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Hort, Tai An 271018, Peoples R China

[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

[3] Laiyang Agr Coll, Laiyang 265200, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 16期

关键词：

apricot; cDNA; S-genotype; self-incompatibility; S-RNase;

D O I：

10.1007/s10529-005-0019-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA of 417 bp encoding an S-RNase gene, named PA S3, was isolated from apricot, Prunus aremeniaca. Nine S-alleles, S-1-S-9, were recognized by S-allele-specific PCR and confirmed by Southern blot analysis using PA S3 as probe. The S-genotypes of the six cultivars were determined and the results of self- and cross-pollination tests among the six cultivars were consistent with the predicted S-haplotypes by PCR analysis.

引用

页码：1205 / 1209

页数：5

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