Assessment of genetic diversity in Sudanese maize (Zea mays L.) genotypes using random amplified polymorphic DNA (RAPD) markers

被引:0
|
作者
Abuali, Atif I. [2 ]
Abdelmula, Awadalla A. [3 ]
Khalafalla, Mutasim M. [1 ]
机构
[1] Natl Res Ctr, Commiss Biotechnol & Genet Engn, Khartoum, Sudan
[2] Natl Res Ctr, Desertificat Res Inst, Khartoum, Sudan
[3] Univ Khartoum, Dept Agron, Fac Agr, Shambat 13314, Sudan
来源
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 42期
关键词
Randomly amplified polymorphic DNA (RAPD) markers; DNA polymorphism; maize genetic diversity;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The randomly amplified polymorphic DNA (RAPD) molecular markers were used to assess genetic diversity in 27 Sudanese maize genotypes. Ten primers were used, resulting in the amplification of 59 fragments, of which 53 (89.33) were polymorphic. The maximum number of fragment bands (10) were produced by the primer A-1 with 100% polymorphism, while the minimum numbers of fragments (3) were produced by the primer OPA-20. Using the unweighted pair group method with arithmetic averages (UPGMA) method, the genetic associations obtained showed three distinct heterotic groups. The high rate of polymorphism between genotypes revealed by RAPD markers indicated that the method is efficient to analyze genetic divergence and can be used to establish consistent heterotic groups between maize genotypes.
引用
收藏
页码:8245 / 8250
页数:6
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