Enhancement of L-cysteine production by disruption of yciW in Escherichia coli

被引：37

作者：

Kawano, Yusuke ^{[1
]}

Ohtsu, Iwao ^{[1
]}

Takumi, Kazuhiro ^{[2
]}

Tamakoshi, Ai ^{[1
]}

Nonaka, Gen ^{[2
]}

Funahashi, Eri ^{[1
]}

Ihara, Masaki ^{[3
,4
]}

Takagi, Hiroshi ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300192, Japan

[2] Ajinomoto Co Inc, Res Inst Biosci Prod & Fine Chem, Kawasaki, Kanagawa 2108681, Japan

[3] Shinshu Univ, Fac Agr, Nagano 3994511, Japan

[4] JST, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2015年 / 119卷 / 02期

基金：

日本学术振兴会;

关键词：

Escherichia coli; L-Cysteine fermentation; In silico analysis; CysB-binding motif; yciW gene; SALMONELLA-TYPHIMURIUM; IDENTIFICATION; OVERPRODUCTION; CYSTINE; PROTEIN; REGIONS;

D O I：

10.1016/j.jbiosc.2014.07.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Using in silico analysis, the yciW gene of Eschetichia coil was identified as a novel L-cysteine regulon that may be regulated by the transcriptional activator CysB for sulfur metabolic genes. We found that overexpression of yciW conferred tolerance to L-cysteine, but disruption of yciW increased L-cysteine production in E. coli. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：176 / 179

页数：4

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