Extracellular oxidases of the lignin-degrading fungus Panus tigrinus

被引:10
|
作者
Cadimaliev, DA
Revin, VV
Atykyan, NA
Samuilov, VD
机构
[1] Ogarev Mordovian State Univ, Dept Biotechnol, Fac Biol, Saransk 430000, Russia
[2] Moscow MV Lomonosov State Univ, Biol Fac, Dept Physiol Microorgan, Moscow 119899, Russia
关键词
lignin; biodegradation; oxidase; laccase; white rot fungi; Panus (Lentinus) tigrinus;
D O I
10.1007/s10541-005-0171-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two extracellular oxidases (laccases) were isolated from the extracellular fluid of the fungus Panus (Lentinus) tigrinus cultivated in low-nitrogen medium supplemented with birch sawdust. The enzymes were purified by successive chromatography on columns with TEAE-cellulose and DEAE-Toyopearl 650M. Both oxidases catalyze oxidation of pyrocatechol and ABTS. Moreover, oxidase 1 also catalyzes oxidation of guaiacol, o-phenylenediamine, and syringaidazine. The enzymes have identical pH (7.0) and temperature (60-65 degrees C) optimums. Absorption spectra of the oxidases differ from the spectra of typical "blue" laccases and are similar to the spectrum of yellow oxidase.
引用
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页码:703 / 707
页数:5
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