miRNA-124-3p targeting of LPIN1 attenuates inflammation and apoptosis in aged male rats cardiopulmonary bypass model of perioperative neurocognitive disorders

被引：12

作者：

Han, Jiang ^{[1
]}

Pu, Cui-Xia ^{[1
]}

Xiao, Qiu-Xia ^{[1
]}

Tang, Li-Jia ^{[1
]}

Liu, Ting ^{[1
]}

He, Li ^{[1
]}

Ren, Ying-Bo ^{[1
]}

Liu, Qing ^{[1
]}

Zhang, Ying ^{[1
]}

机构：

[1] Southwest Med Univ, Dept Anesthesiol, Hosp TCM, Luzhou 646000, Sichuan, Peoples R China

来源：

EXPERIMENTAL GERONTOLOGY | 2021年 / 155卷

关键词：

Perioperative neurocognitive disorder; MiR-124-3p; LPIN1; Apoptosis; Inflammation; POSTOPERATIVE COGNITIVE DYSFUNCTION; TRAUMATIC BRAIN-INJURY; MOUSE MODEL; MICRORNAS; CONTRIBUTES; PROGRESSION; AUTOPHAGY; INSIGHTS; DISEASE; ROLES;

D O I：

10.1016/j.exger.2021.111578

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Perioperative neurocognitive disorder (PND) is recently recommended to define the cognitive decrease during the perioperative period. However, the disease's underlying mechanisms remain unclear. MicroRNAs (miRNAs) are noncoding RNAs that play a vital role in regulating neuroregeneration and neuronal apoptosis. In this study, miR-124-3p was significantly reduced in the PND rat model after a cardiopulmonary bypass (CPB) procedure. MicroRNA-124 (miR-124)-3p-overexpressed lentivirus was constructed and injected via the intracerebroventricular method before CPB. Morris Water Maze test (WMW) and the Open-Field test (OFT) were used to measure behavior changes, data shows decline of cognitive function of rats after CPB. PND rats expressed higher A beta and p-Tau Protein by using immunohistochemistry (IHC) analyses and Enzyme-Linked Immune Sorbent Assay (ELISA). Moreover, the results of IHC, ELISA, Western Blot analysis (WB) and Terminaldeoxynucleotidyl Transferase Mediated Nick End Labeling Assay (TUNEL) showed CPB procedure induced inflammation and apoptosis in rats with PND. The data also revealed the protective function of miR-124-3p overexpression against PND in relieving inflammation, cell apoptosis, and alleviating repaired cognitive function. Moreover, miR-124-3p was predicted by directly targeting LPIN1. This study gives a novel viewpoint that miR-124-3p could improve the state of PND via modulating LPIN1, therefore providing a new strategy for preventing and treating PND in a preclinical application.

引用

页数：10