Processing of DNA Double-Strand Breaks in Yeast

被引：8

作者：

Gnugge, Robert ^{[1
]}

Oh, Julyun ^{[1
]}

Symington, Lorraine S. ^{[1
]}

机构：

[1] Columbia Univ, Med Ctr, New York, NY 10027 USA

来源：

MECHANISMS OF DNA RECOMBINATION AND GENOME REARRANGEMENTS: METHODS TO STUDY HOMOLOGOUS RECOMBINATION | 2018年 / 600卷

关键词：

DAMAGE CHECKPOINT ACTIVATION; END RESECTION; RECOMBINATION; ACCUMULATION; PATHWAY; GENES; EXO1;

D O I：

10.1016/bs.mie.2017.11.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-stranded DNA (ssDNA) intermediates are essential for homology-dependent repair of DNA double-strand breaks (DSBs) and for the DNA damage response. Here we describe methods routinely used to identify ssDNA intermediates formed by end processing of site-specific DSBs in Saccharomyces cerevisiae. These methods have been applied in other model systems and human cell lines, and are useful tools to gain insight into the enzymes that process DSBs and how they are regulated.

引用

页码：1 / 24

页数：24

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