Development of EST-SSR primers and their practicability test for Laminaria

被引：11
|
作者：
Wang Guoliang [1 ,2 ]
Tan Xiaolong [1 ]
Shen Junling [3 ]
Li Jian [1 ]
Zhang Lei [1 ]
Sun Jianwei [2 ]
Wang Bin [2 ]
Weng Manli [2 ]
Liu Tao [1 ]
机构：
[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
[2] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
[3] Qingdao Agr Univ, Coll Hort, Qingdao 266109, Peoples R China
来源：
ACTA OCEANOLOGICA SINICA | 2011年 / 30卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Laminaria; EST-SSRs; germplasm identification; polymorphism analysis; EXPRESSED SEQUENCE TAGS; MARKERS; PLANTS; MICROSATELLITES;
D O I：
10.1007/s13131-011-0125-4
中图分类号：
P7 [海洋学];
学科分类号：
0707 ;
摘要：
Laminaria (Saccharina) is one of the economic important seaweeds. Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software, two hundred and fifty-four SSRs in 206 ESTs were found. From the 254 SSRs, sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most (more than 80%) of the 12 Laminara DNA templates. Genetic diversity study of 12 Laminaria lines, which were widely used in breeding and economic cultivation in China, was performed based on the obtained SSR data.
引用
收藏
页码：112 / 117
页数：6
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