Serological diagnosis of syphilis:: Enzyme-linked immunosorbent assay to measure antibodies to individual recombinant Treponema pallidum antigens

被引：9

作者：

Martin, Irene E. ^{[1
]}

Lau, Allan ^{[1
]}

Sawatzky, Pam ^{[1
]}

Tsang, Raymond S. W. ^{[1
]}

Cuff, Wilfred ^{[2
]}

Lee, Craig ^{[3
]}

MacPherson, Paul A. ^{[3
]}

Mazzulli, Tony ^{[4
]}

机构：

[1] Publ Hlth Agcy Canada, Pathogen Neisseria & Syphilis Diagnost Sect, Natl Microbiol Lab, Winnipeg, MB R3E 3R2, Canada

[2] Publ Hlth Agcy Canada, Div Math Biol & Bioinformat, Natl Microbiol Lab, Winnipeg, MB R3E 3R2, Canada

[3] Ottawa Gen Hosp, Ottawa, ON K1H 8L6, Canada

[4] Mt Sinai Hosp, Dept Microbiol, Toronto, ON M5G 1X5, Canada

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2008年 / 29卷 / 02期

关键词：

syphilis; recombinant antigens; EIA;

D O I：

10.1080/15321810801887771

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We standardized an indirect ELISA for measurement of serum antibody levels to four individual treponemal recombinant proteins that have been commonly used in a number of commercial EIAs, mostly as a mixture of antigens. When tested with 127 syphilis-negative and 37 secondary syphilis sera, ELISA O.D.s obtained for each of the four antigens clearly distinguished between these two groups of samples. Sensitivity and specificity of 100% was obtained with the current set of samples. Further evaluations with sera from different stages of syphilis can help to define the applications of this ELISA test for each of the four antigens studied.

引用

页码：143 / 151

页数：9

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