Induction of apoptosis in human colon carcinoma COLO 205 cells by the recombinant α subunit of C-phycocyanin

被引：10
|
作者：
Lu, Weihong [1 ,2 ]
Yu, Ping [1 ]
Li, Jianrong [1 ]
机构：
[1] Zhejiang Gongshang Univ, Coll Food Sci, Hangzhou 310035, Zhejiang, Peoples R China
[2] Jinhua Coll Profess & Technol, Sch Med, Jinhua 321000, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 03期
关键词：
C-phycocyanin alpha subunit; COLO; 205; Apoptosis; Cytochrome c release; Caspase activation; SPIRULINA-PLATENSIS; NEUROBLASTOMA; GENERATION;
D O I：
10.1007/s10529-010-0464-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The alpha-subunit of C-phycocyanin (CpcA) was expressed in Escherichia coli and purified. The recombinant CpcA inhibited the growth of human colon carcinoma COLO 205 cells. Typical apoptotic morphological characteristics, such as chromatin condensation and nuclear fragmentation, were observed in CpcA-treated COLO 205 cells by fluorescence microscopy and transmission electron microscopy. Moreover, the apoptotic process was associated with the Bax/Bcl-2 ratio up-regulation, mitochondrial membrane depolarization, cytochrome c release, and caspase-9 activation. These findings indicate that CpcA induced the death of COLO 205 cells through the intrinsic apoptotic pathway.
引用
收藏
页码：637 / 644
页数：8
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