Breast Cancer Targeting Novel microRNA-Nanoparticles for Imaging

MicroRNAs (miRNAs) are one of the most prevalent small (similar to 22 nucleotide) regulatory RNA classes in animals. These miRNAs constitute nearly one percent of genes in the human genome, making miRNA genes one of the more abundant types of regulatory molecules. MiRNAs have been shown to play important roles in cell development, apoptosis, and other fundamental biological processes. MiRNAs exert their influence through complementary base-pairing with specific target mRNAs, leading to degradation or translational repression of the targeted mRNA. We have identified and tested a novel microRNA (miR-491) and demonstrated increased apoptosis in hepatocellular carcinoma cells (HepG2) and in human breast cancer cells (HBT3477) in vitro. We prepared a novel cancer targeting assembly of gold nanoparticles (GNP) with Quantum dots, miR-491, and MAb-ChL6 coupled through streptavidin/biotin for effective transfection, and to induce apoptosis in specific cancer cells for imaging and targeted therapy. The targeting and apoptosis inducing ability was tested by confocal and electron microscopy. The MAb-GNP-miR491-Qdot construct effectively transfected into the HBT3477 cells and induced apoptosis the confirmation of these results would suggest a new class of molecules for the imaging and therapy of breast cancer.