Direct electrochemistry and immobilization of glucose oxidase on carbon black

The IR spectroscopic and electrochemical measurements demonstrated that glucose oxidase (GOx) could be immobilized on the surface of carbon black (CB) using a simple adsorption method. The electrochemical measurements indicated that GOx immobilized on CB could undergo the quasi-reversible and direct electrochemical reaction and keep the bioelectrocatalytic activity for the glucose oxidation. Even after conservation for two weeks, its electrocatalytic activity decreased only 5%, illustrating the good stability of GOx immobilized on CB.