The deletion of the ORF1 and ORF71 genes reduces virulence of the neuropathogenic EHV-1 strain Ab4 without compromising host immunity in horses

The equine herpesvirus type 1 (EHV-1) ORF1 and ORF71 genes have immune modulatory effects in vitro. Experimental infection of horses using virus mutants with multiple deletions including ORF1 and ORF71 showed promise as vaccine candidates against EHV-1. Here, the combined effects of ORF1 and ORF71 deletions from the neuropathogenic EHV-1 strain Ab4 on clinical disease and host immune response were further explored. Three groups of EHV-1 naive horses were experimentally infected with the ORF1/71 gene deletion mutant (Ab4 Delta ORF1/71), the parent Ab4 strain, or remained uninfected. In comparison to Ab4, horses infected with Ab4 Delta ORF1/71 did not show the initial high fever peak characteristic of EHV-1 infection. Ab4 Delta ORF1/71 infection had reduced nasal shedding (1/5 vs. 5/5) and, simultaneously, decreased intranasal interferon (IFN)-alpha, interleukin (IL)-10 and soluble CD14 secretion. However, Ab4 and Ab4 Delta ORF1/71 infection resulted in comparable viremia, suggesting these genes do not regulate the infection of the mononuclear cells and subsequent viremia. Intranasal and serum anti-EHV-1 antibodies to Ab4 Delta ORF1/71 developed slightly slower than those to Ab4. However, beyond day 12 post infection (d12pi) serum antibodies in both virus-infected groups were similar and remained increased until the end of the study (d114pi). EHV-1 immunoglobulin (Ig) G isotype responses were dominated by short-lasting IgG1 and long-lasting IgG4/7 antibodies. The IgG4/7 response closely resembled the total EHV-1 specific antibody response. Ex vivo re-stimulation of PBMC with Ab4 resulted in IFN-gamma and IL-10 secretion by cells from both infected groups within two weeks pi. Flow cytometric analysis showed that IFN-gamma producing EHV-1-specific T-cells were mainly CD8(+)/IFN-gamma(+) and detectable from d32pi on. Peripheral blood IFN-gamma(+) T-cell percentages were similar in both infected groups, albeit at low frequency (similar to 0.1%). In summary, the Ab4 Delta ORF1/71 gene deletion mutant is less virulent but induced antibody responses and cellular immunity similar to the parent Ab4 strain.