Cytotoxic effects of the mycotoxin beauvericin to human cell lines of myeloid origin

被引:56
|
作者
Caló, L
Fornelli, F
Ramires, R
Nenna, S
Tursi, A
Caiaffa, MF
Macchia, L
机构
[1] Univ Bari, Policlin, Dept Clin Immunol & Allergol, I-70124 Bari, Italy
[2] Univ Foggia, Fac Med, I-71100 Foggia, Italy
关键词
cell line models; cytotoxicity; food contaminants; mycotoxins; myeloid lineage;
D O I
10.1016/j.phrs.2003.07.002
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Beauvericin, a cyclic hexadepsipeptide of potential importance to the health of humans and domestic animals, has been reported to exert cytotoxic effects on several mammalian cell types and to induce apoptosis. We investigated the cytotoxicity of this compound to two human cell lines of myeloid origin: the monocytic lymphoma cells U-937 and the promyelocytic leukemia cells HL-60. In some experiments HL-60 cells partially differentiated towards the eosinophilic phenotype were also used. Cultures of U-937 cells and HL-60 cells in stationary phase were exposed to beauvericin at concentrations ranging from 100nM to 300 muM for periods of time of 4 and 24 h, respectively. The effects of beauvericin on cell viability were assessed by the Trypan blue exclusion method. In another set of experiments, performed with U-937 cells, the mycotoxin was included in the culture medium at passaging, in order to assess its possible effects on cell growth. Viability of both U-937 cells and HL-60 cells was not affected by beauvericin at concentrations up to 3 muM, after 4 h exposure, whereas a steady decline was seen at higher concentrations. Similarly, after an exposure time of 24 h, a decline in viability was observed in cultures exposed to beauvericin at a concentration of 10 muM or higher. Thus, 50% cytotoxic concentrations at 24 h of congruent to30 muM and congruent to15 muM were estimated for U-937 cells and HL-60 cells, respectively. Similar experiments were performed with cultures of HL-60 cells partially differentiated towards the eosinophilic phenotype, revealing that, in 4 h exposure experiments (but not in 24 h experiments), the viability of these cultures underwent a significantly less pronounced decline, in comparison to undifferentiated HL-60 cultures. Interestingly, when U-937 cells were allowed to proliferate in the presence of the mycotoxin, included in the culture medium at passaging, a substantial cytotoxicity was observed at lower concentrations, compared with prevalently resting, stationary phase cultures. Accordingly, a definite inhibition of the proliferative capability of the cells was detected. The information provided by this work may be useful in selecting appropriate myeloid cell models for the development of biossays aimed at detecting beauvericin (and, possibly, other mycotoxins) in foods and other commodities. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:73 / 77
页数:5
相关论文
共 50 条
  • [1] Cytotoxic effects of the mycotoxin beauvericin to human cell lines of myeloid origin (vol 49, pg 73, 2004)
    Calò, L
    Fornelli, F
    Ramires, R
    Nenna, S
    Tursi, A
    Caiaffa, MF
    Macchia, L
    PHARMACOLOGICAL RESEARCH, 2004, 49 (03) : 299 - 299
  • [2] Cytotoxic effects of high concentrations of sodium ascorbate on human myeloid cell lines
    Domenico Mastrangelo
    Lauretta Massai
    Francesco Lo Coco
    Nélida Inés Noguera
    Loredana Borgia
    Giuseppe Fioritoni
    Anna Berardi
    Antonio Iacone
    Michela Muscettola
    Elvira Pelosi
    Germana Castelli
    Ugo Testa
    Francesco Di Pisa
    Giovanni Grasso
    Annals of Hematology, 2015, 94 : 1807 - 1816
  • [3] Cytotoxic effects of high concentrations of sodium ascorbate on human myeloid cell lines
    Mastrangelo, Domenico
    Massai, Lauretta
    Lo Coco, Francesco
    Noguera, Nelida Ines
    Borgia, Loredana
    Fioritoni, Giuseppe
    Berardi, Anna
    Iacone, Antonio
    Muscettola, Michela
    Pelosi, Elvira
    Castelli, Germana
    Testa, Ugo
    Di Pisa, Francesco
    Grasso, Giovanni
    ANNALS OF HEMATOLOGY, 2015, 94 (11) : 1807 - 1816
  • [4] Auraptene-induced cytotoxic effects in acute myeloid leukemia cell lines
    Ghorbani, Majid
    Soukhtanloo, Mohammad
    Farrokhi, Amir Salek
    Hassanian, Seyed Mahdi
    Ghorbani, Fatemeh
    Afshari, Amir Reza
    Taherian, Mohsen
    Sadeghian, Mohammad Hadi
    MEDICAL ONCOLOGY, 2023, 40 (08)
  • [5] Effects of the mycotoxin ochratoxin A and some of its metabolites on human kidney cell lines
    Müller, G
    Burkert, B
    Rosner, H
    Köhler, H
    TOXICOLOGY IN VITRO, 2003, 17 (04) : 441 - 448
  • [6] Cytotoxic effects of quinoxaline derivatives on human cancer cell lines
    Yoo, HW
    Lee, YS
    Suh, ME
    Kim, DJ
    Park, SW
    ARCHIV DER PHARMAZIE, 1998, 331 (10) : 331 - 333
  • [7] Cytotoxic effects of sodium phenylbutyrate on human neuroblastoma cell lines
    Pelidis, MA
    Carducci, MA
    Simons, JW
    INTERNATIONAL JOURNAL OF ONCOLOGY, 1998, 12 (04) : 889 - 893
  • [8] Cytotoxic and antiproliferative effects of heptaacetyltiliroside on human leukemic cell lines
    Dimas, K
    Demetzos, C
    Vaos, B
    Marselos, M
    Kokkinopoulos, D
    LEUKEMIA RESEARCH, 1999, 23 (11) : 1021 - 1033
  • [9] Cytotoxic effects of violacein in human uveal melanoma cell lines
    Saraiva, VS
    Marshall, JC
    Cools-Lartigue, J
    Burnier, MN
    MELANOMA RESEARCH, 2004, 14 (05) : 421 - 424
  • [10] Cytotoxic and Photocytotoxic Effects of Cercosporin on Human Tumor Cell Lines
    Mastrangelopoulou, Maria
    Grigalavicius, Mantas
    Berg, Kristian
    Menard, Mathilde
    Theodossiou, Theodossis A.
    PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2019, 95 (01) : 387 - 396