Investigation of Red Blood Cells by Atomic Force Microscopy

被引：15

作者：

Sergunova, Viktoria ^{[1
]}

Leesment, Stanislav ^{[2
]}

Kozlov, Aleksandr ^{[3
]}

Inozemtsev, Vladimir ^{[1
]}

Platitsina, Polina ^{[4
]}

Lyapunova, Snezhanna ^{[1
]}

Onufrievich, Alexander ^{[5
]}

Polyakov, Vyacheslav ^{[2
]}

Sherstyukova, Ekaterina ^{[1
,3
]}

机构：

[1] VA Negovsky Res Inst Gen Reanimatol, Fed Res & Clin Ctr Intens Care Med & Rehabilitol, Lab Biophys Cell Membranes Crit State, Moscow 107031, Russia

[2] NT MDT Spectrum Instruments, Proezd 4922,4-3 Zelenograd, Zelenograd 124460, Russia

[3] Sechenov Univ, Sechenov First Moscow State Med Univ, Dept Med & Biol Phys, Moscow 119991, Russia

[4] Inst Biotechn Syst & Technol Natl Res MIET, Shokin Sq Build1, Zelenograd 124498, Russia

[5] Minist Def Russian Federat, NN Burdenko Main Mil Clin Hosp, Fed State Budgetary Inst, Hosp Sq,Build 3, Moscow 105094, Russia

来源：

SENSORS | 2022年 / 22卷 / 05期

关键词：

imaging; atomic force microscopy; red blood cells; morphology; nanostructure; cytoskeleton; membrane stiffness; ERYTHROCYTE CYTOSKELETON; MEMBRANES; NANOSTRUCTURE; VISUALIZATION; STIFFNESS;

D O I：

10.3390/s22052055

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Currently, much research is devoted to the study of biological objects using atomic force microscopy (AFM). This method's resolution is superior to the other non-scanning techniques. Our study aims to further emphasize some of the advantages of using AFM as a clinical screening tool. The study focused on red blood cells exposed to various physical and chemical factors, namely hemin, zinc ions, and long-term storage. AFM was used to investigate the morphological, nanostructural, cytoskeletal, and mechanical properties of red blood cells (RBCs). Based on experimental data, a set of important biomarkers determining the status of blood cells have been identified.

引用

页数：13

共 50 条

[1] Atomic force microscopy: From red blood cells to immunohaematology
Yeow, Natasha
Tabor, Rico F.
Garnier, Gil
[J]. ADVANCES IN COLLOID AND INTERFACE SCIENCE, 2017, 249 : 149 - 162
[2] Diseased red blood cells studied by atomic force microscopy
Chen, Yong
Cai, Jiye
Zhao, Jingxian
[J]. International Journal of Nanoscience, Vol 1, Nos 5 and 6, 2002, 1 (5-6): : 683 - 688
[3] Diseased red blood cells studied by atomic force microscopy
Chen, Y
Cai, JY
Zhao, JX
[J]. ASIANANO 2002, PROCEEDINGS, 2003, : 309 - 314
[4] Quantification of red blood cells using atomic force microscopy
O'Reilly, M
McDonnell, L
O'Mullane, J
[J]. ULTRAMICROSCOPY, 2001, 86 (1-2) : 107 - 112
[5] Nanodiagnostics performed on human red blood cells with atomic force microscopy
Hekele, O.
Goesselsberger, C. G.
Gebeshuber, I. C.
[J]. MATERIALS SCIENCE AND TECHNOLOGY, 2008, 24 (09) : 1162 - 1165
[6] Imaging the surface details of red blood cells with atomic force microscopy
Nowakowski, R
Luckham, P
[J]. SURFACE AND INTERFACE ANALYSIS, 2002, 33 (02) : 118 - 121
[7] Investigation of the influence of phenosan, ichphan-10, and melafen on red blood cells in vivo by atomic force microscopy
V. I. Binyukov
O. M. Alekseeva
E. M. Mil
A. A. Albantova
S. G. Fattachov
A. N. Goloshchapov
E. B. Burlakova
A. I. Konovalov
[J]. Doklady Biochemistry and Biophysics, 2011, 441 : 245 - 247
[8] Investigation of the Influence of Phenosan, Ichphan-10, and Melafen on Red Blood Cells in vivo by Atomic Force Microscopy
Binyukov, V. I.
Alekseeva, O. M.
Mil, E. M.
Albantova, A. A.
Fattachov, S. G.
Goloshchapov, A. N.
Burlakova, E. B.
Konovalov, A. I.
[J]. DOKLADY BIOCHEMISTRY AND BIOPHYSICS, 2011, 441 (01) : 245 - 247
[9] Probing spectrin organization in red blood cells using microfluidics and atomic force microscopy
Pagani, I
LeDuc, P
[J]. BIOPHYSICAL JOURNAL, 2005, 88 (01) : 265A - 265A
[10] Atomic force microscopy study of fine structures of the entire surface of red blood cells
Zhang, PC
Bai, CL
Huang, YM
Zhao, H
Fang, Y
Wang, NX
Li, Q
[J]. SCANNING MICROSCOPY, 1995, 9 (04) : 981 - 988

← 1 2 3 4 5 →