This study was aimed at evaluating the in vitro degradation, the in vivo biocompatibility and at comparing the effects of two methods of sterilization on poly(L-lactic acid) (PLA(94)) resorbable mesh. The mesh was manufactured to be used as surgical soft tissue reinforcement in the vaginal area. Samples of 100 mg of PLA(94) mesh (10 x 10 mm(2)) were immersed in isoosmolar 0.13M, pH 7.4 phosphate buffer solution at 37 degrees C, during 12 months. The hydrolytic degradation up to 12 months after immersion was monitored by measuring weight loss, mesh area changes, and by various analytical techniques namely Differential scanning calorimetry (DSC), capillary zone electrophoresis (CZE), size exclusion chromatography (SEC), and environmental scanning electron microscopy (ESENI). Specimens of nonsterilized, ethylene-oxide (ETO) sterilized, and gamma-ray sterilized PLA(94) mesh were compared. Fifteen samples were implanted in an incisional hernia Wistar rat model. Histopathology was performed up to 90 days after implantation to evaluate the inflammatory response and the collagen deposition. Although the decrease of molecular weight due to polymer chain scissions started 6 weeks after in vitro immersion, water-soluble degradation products and decrease of tensile strength appeared after 8 months only. Analyses showed also that ETO sterilization did not affect the degradation of the PLA(94) mesh. In contrast, gamma-ray sterilization increased very much the sensitivity of the mesh to the hydrolytic degradation. In vivo, the PLA(94) mesh exhibited good biocompatibility over the investigated time period. ((C) 2007 Wiley Periodicals, Inc.
机构:
Xinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R China
Xinxiang Med Univ, Sch Life Sci & Technol, Key Lab Biomed Mat, Xinxiang 453003, Peoples R ChinaXinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R China
Wang, Peng
Wang, Mengyuan
论文数: 0引用数: 0
h-index: 0
机构:
Xinxiang Med Univ, Sch Life Sci & Technol, Key Lab Biomed Mat, Xinxiang 453003, Peoples R ChinaXinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R China
Wang, Mengyuan
Wei, Xiangjuan
论文数: 0引用数: 0
h-index: 0
机构:
Xinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R ChinaXinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R China
Wei, Xiangjuan
论文数: 引用数:
h-index:
机构:
Xie, Liqin
Tian, Linqiang
论文数: 0引用数: 0
h-index: 0
机构:
Xinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R ChinaXinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R China
Tian, Linqiang
Yang, Zhijun
论文数: 0引用数: 0
h-index: 0
机构:
Xinxiang Med Univ, Sch Life Sci & Technol, Key Lab Biomed Mat, Xinxiang 453003, Peoples R ChinaXinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R China
Yang, Zhijun
Zhou, Zhimin
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Med Sci & Peking Union Med Coll, Inst Biomed Engn, Biomed Barriers Res Ctr, Tianjin 300192, Peoples R ChinaXinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R China
Zhou, Zhimin
Chen, Hongli
论文数: 0引用数: 0
h-index: 0
机构:
Xinxiang Med Univ, Sch Life Sci & Technol, Key Lab Biomed Mat, Xinxiang 453003, Peoples R China
Xinxiang Med Univ, Sch Life Sci & Technol, Key Lab Biomed Mat, 601 Jinsui Rd, Xinxiang 453003, Peoples R ChinaXinxiang Med Univ, Affiliated Hosp 3, Dept Orthoped Surg, Xinxiang 453000, Peoples R China
机构:
Sungkyunkwan Univ, Dept Med Device Management & Res, SAIHST, Seoul, South KoreaSungkyunkwan Univ, Dept Med Device Management & Res, SAIHST, Seoul, South Korea
Yoon, Sang-Don
论文数: 引用数:
h-index:
机构:
Kwon, Young-Sam
Lee, Kyu-Sung
论文数: 0引用数: 0
h-index: 0
机构:
Sungkyunkwan Univ, Dept Med Device Management & Res, SAIHST, Seoul, South Korea
Sungkyunkwan Univ, Dept Urol, Samsung Med Ctr, Sch Med, 81 Irwon Ro, Seoul 06351, South KoreaSungkyunkwan Univ, Dept Med Device Management & Res, SAIHST, Seoul, South Korea