Differentiation of Mycoplasma species by 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis fingerprinting

被引：54

作者：

McAuliffe, L

Ellis, RJ

Ayling, RD

Nicholas, RAJ

机构：

[1] Vet Labs Agcy Weybridge, Dept Bacterial Dis, Mycoplasma Grp, Addlestone KT15 3NB, Surrey, England

[2] Univ London Imperial Coll Sci Technol & Med, NERC, Ctr Populat Biol, Ascot SL5 7PY, Berks, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 10期

关键词：

D O I：

10.1128/JCM.41.10.4844-4847.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE. This method could enable the rapid identification of many mycoplasma species for which there is no specific PCR available and which are currently identified by using culture and serological tests.

引用

页码：4844 / 4847

页数：4

共 50 条

[1] 16S rDNA PCR and denaturing gradient gel electrophoresis;: a single generic test for detecting and differentiating Mycoplasma species
McAuliffe, L
Ellis, RJ
Lawes, JR
Ayling, RD
Nicholas, RAJ
[J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (08) : 731 - 739
[2] The detection of Mycoplasma (formerly Eperythrozoon) wenyonii by 16S rDNA PCR and denaturing gradient gel electrophoresis
McAuliffe, Laura
Lawes, Joanna
Bell, Suzanna
Barlow, Alex
Ayling, Roger
Nicholas, Robin
[J]. VETERINARY MICROBIOLOGY, 2006, 117 (2-4) : 292 - 296
[3] Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers
Davies, CE
Hill, KE
Wilson, MJ
Stephens, P
Hill, CM
Harding, KG
Thomas, DW
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) : 3549 - 3557
[4] Profiling of bacterial species associated with haddock larviculture by PCR amplification of 16S rDNA and denaturing gradient gel electrophoresis
Griffiths, S
Melville, K
Cook, M
Vincent, S
St Pierre, M
Lanteigne, C
[J]. JOURNAL OF AQUATIC ANIMAL HEALTH, 2001, 13 (04) : 355 - 363
[5] 16S ribosomal DNA-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by PCR and denaturing gradient gel electrophoresis
Kawai, M
Matsutera, E
Kanda, H
Yamaguchi, N
Tani, K
Nasu, M
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (02) : 699 - 704
[6] Effect of fermentable carbohydrates on piglet faecal bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA
Konstantinov, SR
Zhu, WY
Williams, BA
Tamminga, S
de Vos, WM
Akkermans, ADL
[J]. FEMS MICROBIOLOGY ECOLOGY, 2003, 43 (02) : 225 - 235
[7] 16S rRNA GENE-BASED IDENTIFICATION OF BACTERIA IN POSTOPERATIVE ENDOPHTHALMITIS BY PCR-DENATURING GRADIENT GEL ELECTROPHORESIS (PCR-DGGE) FINGERPRINTING
Navarro-Noya, Yendi
Hernandez-Rodriguez, Cesar
Zenteno, Juan C.
Buentello-Volante, Beatriz
Cancino-Diaz, Mario E.
Jan-Roblero, Janet
Cancino-Diaz, Juan C.
[J]. BRAZILIAN JOURNAL OF MICROBIOLOGY, 2012, 43 (01) : 283 - 287
[8] Effect of disodium fumarate on ruminal metabolism and rumen bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA
Mao, S. Y.
Zhang, G.
Zhu, W. Y.
[J]. ANIMAL FEED SCIENCE AND TECHNOLOGY, 2008, 140 (3-4) : 293 - 306
[9] Molecular identification of bacteria from a coculture by denaturing gradient gel electrophoresis of 16S ribosomal DNA fragments as a tool for isolation in pure cultures
Teske, A
Sigalevich, P
Cohen, Y
Muyzer, G
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (11) : 4210 - 4215
[10] 16S rDNA PCR-denaturing gradient gel electrophoresis in detennining proportions of coexisting Actinobacillus actinomycetemcomitans strains
Ihalin, Riikka
Asikainen, Sirkka
[J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 65 (03) : 417 - 424

← 1 2 3 4 5 →