Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae

被引：49
|
作者：
Nayak, S. [1 ]
Singh, S. K. [1 ]
Ramaiah, N. [1 ]
Sreepada, R. A. [1 ]
机构：
[1] Natl Inst Oceanog, Council Sci & Ind Res, Div Biol Oceanog, Panaji 403004, Goa, India
来源：
FISH & SHELLFISH IMMUNOLOGY | 2010年 / 29卷 / 03期
关键词：
Shrimp diseases; Penaeus monodon; Vibrio harveyi; EST; SSH; qPCR; HISTAMINE-RELEASING-FACTOR; DIFFERENTIALLY EXPRESSED GENES; MOLECULAR-IDENTIFICATION; SHRIMP; PROTEIN; HEMOCYANIN; HEMOLYMPH; CLONING; DISCOVERY; JAPONICUS;
D O I：
10.1016/j.fsi.2010.05.010
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
A subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 hypothetical proteins were identified, a few being first reports from shrimps. The most abundant immune relevant genes were ferritin, hemocyanin, and TCTP (translationally controlled tumor protein) indicating their upregulation as also confirmed through qPCR. Post-infection qPCR analyses confirmed 2.04, 2 09, 3 28, 5.49, 647, and 11.63 fold rise respectively in ferritin, penaeidin, MnSOD, lysozyme, TCTP, and hemocyanin genes. These genes may be involved in the regulation of the host defense against V harveyi (C) 2010 Elsevier Ltd All rights reserved
引用
收藏
页码：544 / 549
页数：6
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