High-speed CCD movie camera with random pixel selection, for neurobiology research

被引:0
|
作者
Potter, SM
Mart, AN
Pine, J
机构
关键词
potentiometric probe; voltage-sensitive dye; optical recording; high-speed programmable CCD; neural imaging;
D O I
暂无
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We have designed and built a CCD camera capable of producing movies at over 1000 frames per second. For maximum frame rate, we have incorporated the ability to digitize only the pixels of interest, user-selectable from a custom LabView interface. With a resolution of 64x64 pixels, the system is intended to bridge the gap between fast, small photodiode arrays, and slow, high-resolution scientific CCD cameras. The system was designed for imaging neurons labeled with voltage-sensitive dyes, to allow the simultaneous recording of neural activity in a large number of neurons, or to probe the membrane voltage at many different points of a single neuron.
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页码:243 / 253
页数:11
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