The microglial NLRP3 inflammasome is involved in human SARS-CoV-2 cerebral pathogenicity: A report of three post-mortem cases

被引：29

作者：

Falcon Cama, Viviana ^{[1
,8
]}

Marin-Prida, Javier ^{[2
]}

Acosta-Rivero, Nelson ^{[3
]}

Acosta, Emilio F. ^{[4
,8
]}

Oramas Diaz, Leonardo ^{[1
]}

Casadesus, Ana, V ^{[5
]}

Fernandez-Marrero, Briandy ^{[5
]}

Gilva-Rodriguez, Nathalie ^{[1
]}

Cremata-Garcia, Daina ^{[1
]}

Cervantes-Llanos, Majel ^{[1
]}

Piniella-Matamoros, Beatriz ^{[1
]}

Sanchez, Daisy ^{[4
]}

del Rosario-Cruz, Leticia ^{[6
]}

Borrajero, Israel ^{[7
]}

Diaz, Angelina ^{[4
]}

Gonzalez, Yorexis ^{[4
]}

Penton-Arias, Eduardo ^{[1
,8
]}

Montero-Gonzalez, Teresita ^{[6
]}

Guillen-Nieto, Gerardo ^{[1
,8
]}

Penton-Rol, Giselle ^{[1
,8
]}

机构：

[1] Ctr Genet Engn & Biotechnol CIGB, Ave 31 E-158 & 190,POB 6162, Havana, Cuba

[2] Univ Havana, Inst Pharm & Food, Ctr Res & Biol Evaluat, Ave 23 E-214 & 222,POB 430, Havana, Cuba

[3] Univ Havana, Fac Biol, Ctr Prot Res, Dept Biochem, Havana, Cuba

[4] Ctr Adv Studies Cuba, Havana, Cuba

[5] Ctr Mol Immunol, Direct Immunol & Immunotherapy, Havana, Cuba

[6] Hosp Mil Cent Dr Luis Diaz Soto, Ave Monumental Km 2, Havana, Cuba

[7] Hosp Clin Quirarg Hermanos Ameijeiras, Havana, Cuba

[8] Latin Amer Sch Med, Carretera Panamer Km 3 1-2, Havana 11600, Cuba

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2021年 / 361卷

关键词：

COVID-19; SARS-CoV-2; NLRP3; inflammasome; Macrophages; Human brain; ACTIVATION; COVID-19;

D O I：

10.1016/j.jneuroim.2021.577728

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We herein report, by using confocal immunofluorescence, the colocalization of the SARS-CoV-2 nucleocapsid within neurons, astrocytes, oligodendrocytes and microglia in three deceased COVID-19 cases, of between 78 and 85 years of age at death. The viral nucleocapsid was detected together with its ACE2 cell entry receptor, as well as the NLRP3 inflammasome in cerebral cortical tissues. It is noteworthy that NLRP3 was colocalized with CD68 + macrophages in the brain and lung of the deceased, suggesting the critical role of this type of inflammasome in SARS-CoV-2 lesions of the nervous system/lungs and supporting its potential role as a therapeutic target.

引用

页数：6

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